中国康复理论与实践Issue(1)：32-37,6.DOI:10.3969/j.issn.1006-9771.2016.01.007
丁苯酞注射液对局灶性脑缺血再灌注大鼠的脑保护作用及机制
Effect of Butylphthalide Injection on Neural Protection in Focal Cerebral Ischemia-reperfusion Rats
摘要
Abstract
Objective To observe the neural protection of 3-n-butylphthalide (NBP) injection in focal cerebral ischemia-reperfusion rats. Methods 160 male Sprague-Dawley rats were randomly divided into sham group (n=10), ischemia-reperfusion group (IR group, n=50), high-dose NBP treatment group (high-dose group, n=50), middle-dose NBP treatment group (middle-dose group, n=25) and low-dose NBP treatment group (low-dose group, n=25). The later 4 groups were occluded the middle cerebral artery for 2 hours and reperfused. The sham group was sacrificed 24 hours after operation, and the other groups at 6, 12, 24, 48 and 72 hours after reperfusion, in which 5 of them were stained with TdT mediated dUTP Nick End Labeling (TUNEL) to observe the neuronal apoptosis, and immunohistochemistry to observe the expression of silent information regulation 2 homolog 1 (SIRT1) and peroxisome proliferator-activated receptorγcoactivator-1α(PGC-1α);the other 5 of sham group, IR group and high-dose group were observed with quantitative real-time PCR of SIRT1 and PGC-1α. Results Compared with the IR group, the number of apoptotic cells decreased and the SIRT1 and PGC-1αpositive cells increased in all NBP groups at each time (F>160.60, P<0.001), and it was the least of apoptotic cells and most of SIRT1 and PGC-1α positive cells in the high-dose group (P<0.05), while there was significant difference between the low-dose group and the middle-dose group, excluding 6 hours after reper-fusion (P<0.05). Compared with IR group, the expression of SIRT1 and PGC-1αmRNA increased in the high-dose group at each time (t>4.13, P<0.01). Conclusion NBP can protect brain from apoptosis in focal cerebral ischemia-reperfusion rats, which may relate to more ex-pression of SIRT1 and PGC-1α.关键词
脑缺血再灌注/丁苯酞/凋亡/沉默信息调节因子2相关酶1/过氧化物酶体增殖物激活受体γ辅激活因子1αKey words
cerebral ischemia-reperfuson/Butylphthalide/apoptosis/silent information regulation 2 homolog 1/peroxisome prolifera-tor-activated receptorγcoactivator-1α分类
医药卫生引用本文复制引用
赵秀芹,毛文静,李世英,张晋霞,贺永贵,余红,刘斌..丁苯酞注射液对局灶性脑缺血再灌注大鼠的脑保护作用及机制[J].中国康复理论与实践,2016,(1):32-37,6.基金项目
河北省2013年医学科学研究重点课题计划(No.20130382)。 （No.20130382）
