中国康复理论与实践Issue(1)：38-45,8.DOI:10.3969/j.issn.1006-9771.2016.01.008
深刺加电针对兔膝骨关节炎软骨的影响
Effects of Deep Electroacupuncture on Cartilage in Knee Osteoarthritis Rabbits
摘要
Abstract
Objective To observe the effects of deep electroacupuncture on carlilage tissue in knee osteoarthritis (KOA) rabbits. Meth-ods 40 New Zealand rabbits were randomly divided into normal group (A, n=10) and model group (n=30). The model group was modeled KOA with Hulth-Telhag way, and identified with X-ray. Then they were divided into no-treated group (B, n=10), deep electroacupuncture group (C, n=10) and routine electroacupuncture group (D, n=10) randomly. The groups C and D accepted electroacupuncture since 6 weeks after modeling, for 4 weeks. They were measured with pH of joint fluid, observed structure and pathology of cartilage under transmission electron microscope, detected apoptosis index, and determined the expression of acid-sensing ion channel 1 (ASIC1), p38 mitogen-activated protein kinases (p38MAPK) and p53 with Western blotting, and distribution of ASIC1 with immunohistochemistry in cartilage tissue. Re-sults The pHs of joint fluid from high to low were ranged as the groups A=C>D>B (P<0.01). The cartilage structure was more complete in the groups A, C and D than in the group B. The apoptosis rates from less to more were ranged as the groups A=C<D<B (P<0.01). The ex-pression of ASIC1 and p53 from less to more were ranged as the groups A=C<D<B (P<0.01), while it was A=C<D<B of p38MAPK (P<0.01). Conclusion Deep electroacupuncture can inhibit the expression of ASIC1 and phosphorylation of p38MAPK in chondrocytes, as well as the expression of apoptosis factor p53 in KOA rabbits, result in less apoptosis of chondrocytes and cartilage impairment, which is more ef-fective than routine electroacupuncture.关键词
膝骨关节炎/电针/深刺/凋亡/酸离子敏感通道1/p38丝裂原活化蛋白激酶/p53/兔Key words
knee osteoarthritis/electroacupuncture/deep acupuncture/apoptosis/acid-sensing ion channel 1/p38 mitogen-activated protein kinases/p53/rabbits分类
医药卫生引用本文复制引用
付妮妮,李学智,刘菲,席小芳,任毅,杨晓光,张愉..深刺加电针对兔膝骨关节炎软骨的影响[J].中国康复理论与实践,2016,(1):38-45,8.基金项目
重庆市卫生和计生委中医药科技项目(No.ZY201402117)。 （No.ZY201402117）
