中国康复理论与实践Issue(2)：125-131,7.DOI:10.3969/j.issn.1006-9771.2016.02.001
亚甲基蓝对大鼠局灶性脑缺血再灌注后血脑屏障的保护作用
Effect of Methylene Blue on Blood-brain Barrier after Focal Cerebral Ischemia-reperfusion in Rats
摘要
Abstract
Objective To investigate the protective effect of methylene blue (MB) on blood-brain barrier (BBB) injury after focal cere-bral ischemia-reperfusion in rats. Methods 18 male Sprague-Dawley rats were randomly divided into sham-operated group (n=6), model group (n=6) and MB treatment group (n=6). The left middle cerebral arteries were occluded for 1 hour and reperfused. MB was infused intra-venously immediately after reperfusion (3 mg/kg) and again 2 hours post-reperfusion (1.5 mg/kg), while normal saline was administered in the model group. The sham-operated group was treated as same as the model group without occlusion and infusion. HE staining was used to observe the histological injury in the cortex around the infarcted region 47 hours after reperfusion, while albumin immunohistochemistry was used to evaluate the permeability of the BBB, and immunohistochemistry and double immunofluorescence staining were used to exam-ine the expressions of glial fibrillary acidic protein (GFAP) and aquaporin-4 (AQP-4). Results HE staining showed that cells and blood ves-sels were not intact in the cortex around the infarcted region in the model group and they were better in the MB treatment group. The expres-sions of the albumin, GFAP and AQP-4 were higher in the model group than in the sham-operated group (P<0.01), and were lower in MB treatment group than in the model group (P<0.05). The double immunofluorescence staining showed the colocalization of GFAP and AQP-4 in the astrocytes. Conclusion MB may ameliorate the BBB disruption induced by focal cerebral ischemia-reperfusion through reducing glio-cyte proliferation and down-regulation of AQP-4 expression in rats.关键词
脑缺血再灌注/亚甲基蓝/血脑屏障/胶质原纤维酸性蛋白/水通道蛋白-4/大鼠Key words
cerebral ischemia-reperfusion/methylene blue/blood-brain barrier/glial fibrillary acidic protein/aquaporin-4/rats分类
医药卫生引用本文复制引用
吴敏,方庆,师忠芳,徐立新,董丽萍,闫旭,杨少华,袁芳..亚甲基蓝对大鼠局灶性脑缺血再灌注后血脑屏障的保护作用[J].中国康复理论与实践,2016,(2):125-131,7.基金项目
国家自然科学基金项目(No.81271286 （）
No.81228009)。 （）
