中国康复理论与实践Issue(2)：136-140,5.DOI:10.3969/j.issn.1006-9771.2016.02.003
磷酸化环磷腺苷反应元件结合蛋白对持续惊厥后海马细胞凋亡调控基因表达的影响
Effect of Phosphorylation Cyclic Adenosine Monophosphate Response Element Binding Protein on Expression of Apoptotic Regulated Genes in Hippocampus after Status Convulsivus
摘要
Abstract
Objective To explore the effect of the exogenous phosphorylation cyclic adenosine monophosphate response element bind-ing protein (pCREB) antibody on the expression of apoptotic regulated genes (Bcl-2 and c-Jun) in hippocampus after status convulsivus (SC), to elucidate the role and regulation mechanism of pCREB in convulsive brain injury. Methods Seizures were induced in 24 adult Wi-star rats with lithium-pilocarpine intraperitoneal injection (SC group), another 24 rats were as the normal controls (NC group). Each group was divided into no injection subgroup, normal saline injection subgroup and anti pCREB subgroup according to the injection contents of lat-eral ventricle, with 8 cases in each group. They were sacrificed 6 hours after injection. Both the protein and mRNA expression of Bcl-2 and c-Jun in bilateral hippocampus were detected by immunohistochemistry and in situ hybridization, respectively. Results There was no signifi-cant difference in Bcl-2 protein/mRNA expression among 3 subgroups in the NC group (P>0.05). In the SC group, the expression of Bcl-2 protein/mRNA were lower in the anti pCREB subgroup than in the no injection subgroup and normal saline injection subgroup (P<0.05). There was significant difference in c-Jun protein/mRNA expression among 3 subgroups in both NC group and SC group (P<0.001). The ex-pression of c-Jun protein/mRNA was higher in the normal saline injection subgroup and the anti pCREB subgroup than in the no injection group (P<0.05), especially in the anti pCREB subgroup (P<0.05). Conclusion Exogenous anti-pCREB antibody can down-regulate the ex-pression of Bcl-2 and up-regulate the expression of c-Jun in hippocampal cells after SC.关键词
持续惊厥/磷酸化环磷腺苷反应元件结合蛋白/Bcl-2/c-Jun/大鼠Key words
status convulsivus/phosphorylation cyclic adenosine monophosphate response element binding protein/Bcl-2/c-Jun/rats分类
医药卫生引用本文复制引用
苑爱云,刘秋燕,李小平,侯梅..磷酸化环磷腺苷反应元件结合蛋白对持续惊厥后海马细胞凋亡调控基因表达的影响[J].中国康复理论与实践,2016,(2):136-140,5.基金项目
山东省医药卫生科技发展计划项目(No.2015WS0352)。 （No.2015WS0352）
