中国水稻科学Issue(1)：1-9,9.DOI:10.16819/j.1001-7216.2016.5025
水稻小粒矮秆突变体sgd1（t）的表型分析及基因克隆
Phenotyping and Gene Cloning of a Small-grain Dwarf Mutant sgd1 (t)in Rice
摘要
Abstract
A small-grain dwarf mutant,designated as sgd1 (t ),was identified from the T-DNA insertion mutant lines of Nipponbare.The mutant,genetically stable,was characterized by dwarf plant,small grain,dark green leaves and thick husk.Scanning electron microscope analysis on cell morphology of stem and hull revealed that stem cells in sgd1 (t )failed to form normal cell column and vascular bundles,while epidermal cells with irregular shape in hulls were tightly packed,resulting in confusion in cell arrangement,and sgd1 (t )was a GA-sensitive dwarf mutant.Genetic analysis showed that this trait of dwarfism was controlled by a pair of recessive nuclear gene.Through map-based cloning,the gene sgd1 (t )was mapped in an interval of 230 kb between the markers DF1 3 and DF26 on the short arm of chromosome 9.There was a dwarf gene BC12/GDD1 in this region.Sequence analysis showed that sgd1 (t )had a single-base substitution (G to T)in the fourth exon of the gene,resulting in the replacement of glycine by valine in 186th conservative amino acid.关键词
水稻/小粒矮秆/基因克隆/BC12/GDD1/单碱基突变Key words
rice (Oryza sativa L.)/small grain and dwarfism/gene cloning/BC12/GDD1/single-base substitution分类
生物科学引用本文复制引用
汪鹏,燕海刚,汪国湘,江玲,张文伟,万建民,蔡跃,陈韦韦,马婧,陈新刚,唐小洁,尤小满,孔飞,张杰..水稻小粒矮秆突变体sgd1（t）的表型分析及基因克隆[J].中国水稻科学,2016,(1):1-9,9.基金项目
国家科技支撑计划资助项目(2011BAD35B02-02) （2011BAD35B02-02）
生物种业能力提升计划资助项目([2012]1961) （[2012]1961）
江苏省自主创新课题资助项目[CX(12)1003] （12）
