中国水稻科学Issue(1)：10-16,7.DOI:10.16819/j.1001-7216.2016.5130
水稻无选择标记 Xa21转基因系 CX8621的获得与遗传分析
Generation and Genetic Analysis of Xa21 Transgenic Rice Line CX8621 Without Selectable Markers
摘要
Abstract
Selectable marker-free and stable inheritance are the basic requirements for safe application of transgenic crops.Following the regulations approved by the National Committee for Transgenic Biosafety,the wide spectrum bacterial blight resistance gene Xa21 was transferred into a widely used restorer line Minghui 86 by using a double right-borders T-DNA vector through Agrobacterium tumefaciens-mediated system.The transgene Xa21 was confirmed by resistance identification,PCR analysis and Southern blot in the progenies.The single-copy homozygous transgenic line CX8621 without selectable marker and vector backbone sequences was obtained from T3 generation.The transgenic restorer line CX8621 has stable bacterial blight resistance with years of observation and identification.Up to now, CX8621 has self-fertilized to 1 6th generation with stable inheritance of transgene Xa21 and passed the bio-safety evaluation of small and large scale of field tests (environmental release and productive test).关键词
转基因水稻/白叶枯病/Xa21/无选择标记/稳定遗传Key words
transgenic rice/bacterial blight/Xa21/selectable maker-free/stable inheritance分类
生物科学引用本文复制引用
夏志辉,刘鹏程,高利芬,刘栋峰,姜丽,江光怀,郭乐群,翟文学..水稻无选择标记 Xa21转基因系 CX8621的获得与遗传分析[J].中国水稻科学,2016,(1):10-16,7.基金项目
国家转基因生物新品种培育重大专项(2008/2014ZX08001-002) （2008/2014ZX08001-002）
中国科学院知识创新方向项目(KSCX2-EWN-01) （KSCX2-EWN-01）
