中国水稻科学Issue(1)：17-26,10.DOI:10.16819/j.1001-7216.2016.5092
云南地方稻种抗稻瘟病基因 Pi-d3序列变异分析
Sequence Variation of Pi-d3 for Rice Blast Resistance in Yunnan Rice Landrace
摘要
Abstract
The sequence variations of Pi-d3 for rice blast resistance in 80 Yunnan rice landraces were analyzed by rese-quencing open reading frame (ORF).Compared with Pi-d3 Digu ORF,a total of 39 nucleotide variations were found in 2775-bp ORFs of 80 rice accessions with the average mutation rate of 1 .41 %.The WTBXPi-d3 ORF of 80 rice acces-sions was divided into 37 haplotypes based on the nucleotide variations.H8(28.8%),H4(1 1 .3%)and H23(5 .0%)are high-frequency types,while other types occurred at low frequency.The results showed that the WTBXPi-d3 in Yunnan rice landraces was rich in genetic variations and haplotypes,but low frequency.A total of 28 amino acids variations were found in 33 translation proteins of 37 haplotypes.18 haplotypes with 32 rice landraces including 12 indica accessions possessde the Pi-d3 pseudogenes.The frequency of pseudogene was high.It was possible due to that the Pi-d3 pseudo-gene permeated to indica.Terminators were newly founded at position 32 and 467 of protein except the published termi-nator at position 737.Pi-d3 differed in both type and frequency of haplotypes between the subspecies of indica and ja-ponica,the ecotypes of lowland and upland rice,no-waxy and waxy rice.It indicated that genetic differentiation oc-curred between subspecies or ecotypes.Geographical distribution showed that 37 haplotypes spread over a large area while concentrated in small areas.Puer,Banna and Lincang were the central area with abundant haplotypes,and based on this scale,the types of haplotypes decreased outward progressively.关键词
云南地方稻种/稻瘟病/Pi-d3 基因/单核苷酸多态性Key words
Yunnan rice landraces/rice blast/Pi-d3/single nucleotide polymorphism分类
生物科学引用本文复制引用
杨奕,孙一丁,马继琼,王炎炎,许明辉..云南地方稻种抗稻瘟病基因 Pi-d3序列变异分析[J].中国水稻科学,2016,(1):17-26,10.基金项目
国家自然科学基金资助项目(31360331) （31360331）
科技部基础性工作专项(2012FY110200) （2012FY110200）
云南省自然科学基金资助项目(2013FZ146) （2013FZ146）
