中国水稻科学Issue(1)：27-34,8.DOI:10.16819/j.1001-7216.2016.5101
水稻剑叶角度 qFla-8-2位点的精细定位
Fine Mapping of qFla-8-2 for Flag Leaf Angle in Rice
摘要
Abstract
To better understand the genetic mechanism regulating large rice flag leaf angle,qFla-8 detected previously was further fine-mapped and the candidate genes were predicted.Using a secondary segregating population,composed of 1 72 selfing individual plants with heterozygous region of target gene merely in BC3 F3 population derived from the backcross between 863B (recipient parent)and A7444 (donor parent),genotypes of nine SSR markers in the interval of RM621 5 - RM8265 containing qFla-8 were identified.Combining the phenotypic data, chromosome segment containing qFla-8 was further narrowed and there are two closely linked loci,qFla-8-1 and qFla-8-2 .qFla-8-1 was localized between RM621 5 and RM3 1 53,explaining 22.33% of phenotypic variation,and qFla-8-2 was localized between RM1 309 and RM349 1 ,explaining 23.81 % of phenotypic variation.By developing insertion-deletion (InDel) markers,qFla-8-2 with higher additive effect was finally limited to 67 kb region between InDel marker Z7 and SSR marker RM23071 ,which contains three predicted genes,Os08g0408200 encoding WD40 domain containing protein similar to GAMYB-binding protein, Os08g0408300 encoding hypothetical protein and Os08g0408500 encoding APETALA2-like protein.关键词
水稻/剑叶角度/QTL/精细定位Key words
rice/flag leaf angle/QTL/fine mapping分类
生物科学引用本文复制引用
朱长丰,梁利君,曾思远,李天伟,董冠杉,洪德林..水稻剑叶角度 qFla-8-2位点的精细定位[J].中国水稻科学,2016,(1):27-34,8.基金项目
国家863计划资助项目(2010AA101301) （2010AA101301）
高等学校博士学科点专项科研基金资助项目(20130097110001) （20130097110001）
中央高校基本科研业务费专项资金项目(KYZ201202-9) （KYZ201202-9）
