中国水稻科学Issue(1)：44-52,9.DOI:10.16819/j.1001-7216.2016.5126
籼稻93-11辐射诱变突变体库的创建及其筛选
Construction and Screening of an Irradiation-induced Mutant Library of indica Rice 93-1 1
摘要
Abstract
Mutant libraries are powerful tools for plant germplasm creation and functional analysis of genome.Using 93-1 1 (O .sativa ssp.indica cv.93-1 1 ),an elite variety with complete genome sequences available,we established an irradiation-induced mutant library containing 36 1 7 lines.Three hundred and eight mutant lines were obtained from M2 and M3 generations and classfied into 1 5 types of mutation,including plant height,tiller number,plant architecture, inflorescence architecture,spikelet architecture,fertility,leaf color,leaf shape,heading date,and bentazon resistance. The mutation frequencies ranged from 0.03% to 2.74%.Using reverse genetics approach,we found that CYP704B2 is the causal gene for two male sterile mutants and CYP81A6 is responsible for two bentazon-lethal mutations.For the two male sterile mutants, nucleotide substitution and deletion were detected in the third and fourth exons of CYP704B2 ,respectively.For the two bentazon-lethal mutants,a 7-bp and a 23-bp deletion were detected in the first and second exons of CYP81A6 , respectively.Genetic studies revealed that wild-type plants and mutant plants segregated at 3 ∶ 1 ratio,suggesting that these mutant traits were controlled by single recessive genes.Our study showed that this mutant library is a useful resource for rice genome functional analysis and rice genetic improvement.关键词
籼稻/辐射诱变/突变体库/雄性不育/苯达松敏感Key words
indica/mutagenesis by irradiation/mutant library/male sterility/bentazon-lethal分类
生物科学引用本文复制引用
龙湍,安保光,李新鹏,张维,李京琳,杨瑶华,曾翔,吴永忠,黄培劲..籼稻93-11辐射诱变突变体库的创建及其筛选[J].中国水稻科学,2016,(1):44-52,9.基金项目
海南省重大科技项目(ZDZX2013010) （ZDZX2013010）
