摘要
Abstract
Objective To investigate the study of C21 steroidal glycoside of cynanchum auriculatum on the myoca- rdial protective effect and angiogenesis in chronic heart failure(CHF)rat model and discuss its possible mechanism. Methods CHF Wistar rats models were established and rats were randomly divided into four groups:CHF model group was administered equal volume of normal saline oraly;Low-dose group was administered oraly C21 steroidal glycoside of cynanchum auriculatum according to 0.5 g/kg;Middle-dose group was administered oraly C21 steroidal glycoside of cynanchum auriculatum according to 1.0 g/kg;High-dose group was administered oraly C21 steroidal glycoside of cynanchum auriculatum according to 2.0 g/kg;Another sham group was administered equal volume of normal saline oraly.after 3 weeks,Hemodynamics,cardiac index and microvessel density(MVD) were measured. Western blot and Real-Time PCR were used to detect the expression of stromal cel derived factor-1(SDF-1)protein and mRNA.Results Baishouwu C21 steroidal glycosides and high dose group rats left ventricular systolic pressure (LVSP),mean left ventricular pressure maximum rise and decline rate(±dp/dtmax) was significantly higher than that of the CHF model group,left ventricular diastolic end pressure(LVEDP) was significantly lower than that of the CHF model group,left ventricular relaxation time constant(Tau) was significantly shorter than that of CHF model group,and showed dose dependent,the differences were statisticaly significant (P<0.05);Baishouwu C21 steroidal glycosides dose group rats SDF-1 protein and mRNA expression were significantly higher than those in the CHF model group, and there was a dose dependent,differences were statisticaly significant(P<0.05).Conclusion Baishouwu C21 steroidal glycosides effectively improve heart function of CHF rats.At the same time,it can promote the formation of new blood vessels.The mechanism may and promote the expression of SDF-1.关键词
白首乌C21甾苷/慢性心力衰竭/基质细胞衍生因子-1Key words
C21 steroidal glycoside of cynanchum auriculatum/Chronic heart failure/Angiogenesis/Stromal cel derived factor-1分类
医药卫生
