中国医学创新Issue(34)：8-12,5.DOI:10.3969/j.issn.1674-4985.2015.34.003
肿瘤细胞特异启动子hTERT介导的靶向mdr1基因的RNAi用于逆转卵巢癌细胞MDR的研究
Study of Tumor Cell Specific Promoter hTERT Mediated RNAi Which Targeted mdr1 Gene in Reversion MDR of Ovarian Cancer Cells
摘要
Abstract
Objective:To discuss the feasibility of tumor cell specific promoter hTERT mediated RNAi which targeted mdr1 gene in reversion multidrug resistance(MDR) of ovarian cancer cells.Method:The transcriptional activity of hTERT promoter in ovarian cancer cell line A2780 were verified by the report plasmid carried Luc gene.The hTERT promoter mediated siRNA expression vector phTERT-siMDR1B which targeted mdr1 gene were constructed.Co-transfected inhibition experiment of phTERT-siMDR1B and report plasmid which carried mdr1 gene target sequence was carried out.A2780 cells were furtherly co-transfected by phTERT-siMDR1B expression vector and mdr1 gene expression vector,and the expression level of mRNA and P-gp protein of mdr1 gene in cells were detected.The drug resistance were evaluated by using A2780 cells which has a resistance to Taxol.Result:hTERT promoter had good transcriptional activity in ovarian cancer cell line A2780,whereas without transcriptional activity in normal human diploid cell lines MRC-5. hTERT promoter mediated siMDR1B had good inhibitory effect and specificity.hTERT promoter mediated siMDR1B could significantly inhibit the expression level of mRNA and P-gp protein of mdr1 gene in cells.The drug resistance degree of cells with hTERT promoter mediated siMDR1B to Taxol was significantly reduced.Conclusion:Applying hTERT mediated RNAi which targeted mdr1 gene to reverse MDR of ovarian cancer cells is feasibility.The results of this study may provide an important foundation for the further study of MDR reversal in ovarian cancer.关键词
卵巢癌/多药耐药/hTERT启动子/RNA干扰Key words
Ovarian cancer/Multiple drug resistance/hTERT promoter/RNA interference引用本文复制引用
林燕真,程通,张雅丽,李瑞银,杨连威,温兰玲..肿瘤细胞特异启动子hTERT介导的靶向mdr1基因的RNAi用于逆转卵巢癌细胞MDR的研究[J].中国医学创新,2015,(34):8-12,5.基金项目
福建省卫生厅青年科研课题资助计划项目 （）
