中国执业药师Issue(2)：25-28,4.DOI:10.3969/j.issn.1672-5433.2016.02.006
HPLC测定转化糖电解质注射液中5-羟甲基糠醛含量
Determination of 5-Hydroxymethyl Furfural Content in Invert Sugar and Electrolytes Injection by HPLC
李彩霞 1刘光斌1
作者信息
- 1. 酒钢医院药学部，甘肃 嘉峪关735100
- 折叠
摘要
Abstract
ABSTRACTObjective:To develop a HPLC method for determination of 5-hydroxymethyl furfural content in invert sugar and electrolytes injection.Methods:Kromasil C18 column(4.6 mm×250 mm,5μm)was used, serving acetonitrile-triethylamine solution(9∶91,add 2.5 mL triethylamine to 1 000 mL water,and adjust the pH value to 3.0 with dilute phosphoric acid solution(1→5)as a mobile phase at a flow rate of 1.0 mL/min. The temperature of column was 25 ℃,while the detection wavelength was 284 nm.Results:The standard curve of 5-hydroxymethyl furfural showed a good linearity at a concentration range of 0.002 8~7.0μg/mL(r= 0. 999 8). The average recovery was 99.81%,with a RSD of 1.87%.Conclusion:The developed HPLC method is simple, sensitive,specific and accurate.关键词
转化糖电解质注射液/5-羟甲基糠醛/高效液相色谱法/含量测定Key words
Invert Sugar and Electrolytes Injection/5-Hydroxylmethyl Furfural/HPLC/Content Determination引用本文复制引用
李彩霞,刘光斌..HPLC测定转化糖电解质注射液中5-羟甲基糠醛含量[J].中国执业药师,2016,(2):25-28,4.
