中华神经创伤外科电子杂志Issue(4)：27-34,8.DOI:10.3877/cma.j.issn.2095-9141.2015.04.008
不同穿刺点行子宫内电转对小鼠胚胎皮层发育的影响
Influence of three puncture sites of in utero electroporation on embryonic cortex development
摘要
Abstract
Objective To compare the influence of three different puncture sites of in utero electroporation on the death rate of mice embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis. Methods It groups on the bases of common puncture sites as follows: 1. electric shock only (control); 2. injection near the posterior fontanel, at about one millimeter (here, group A);3. injection along the antero-posterior axis into the cephalic ventricle (group B); and 4. injection near the midpoint between the anterior fontanel and posterior fontanel, at about three millimeters (group C). We compared the four groups using littermate mice, and repeated the experiment with 5 pregnant mice. To detect whether different injection positions affect cell differentiation, cell proliferation, cell migration and cell apoptosis, we stained cells with anti-Tbr1, anti-Tbr2, anti-Pax6 anti-Ctip2, anti- Caspase-3 and anti-Ki67. In differentiation, proliferation, and apoptosis assays, the cell number was analyzed with SPSS 13.0 software, using one way ANOVA. Results We found no statistical significant differences between the three puncture methods in the death rate of embryos, the thickness and the area of cortex, cell differentiation, cell proliferation, cell migration and cell apoptosis (P>0.05). Conclusion Three puncture sites used for in utero electroporation show no significantly different negative impacts during gene transfer into the embryonic mouse brain.关键词
基因转移技术/穿刺点/分化/增殖Key words
Gene transfer techniques/Puncture/Cell differentiation/Cell proliferation引用本文复制引用
张鹏,吴翠莹,陶庆霞,牛力军,陈文锦,刘宁,徐如祥..不同穿刺点行子宫内电转对小鼠胚胎皮层发育的影响[J].中华神经创伤外科电子杂志,2015,(4):27-34,8.基金项目
国家自然科学基金青年项目(项目编号81401031) （项目编号81401031）
