中华神经创伤外科电子杂志Issue(4)：35-39,5.DOI:10.3877/cma.j.issn.2095-9141.2015.04.009
缺氧预处理对颅脑损伤大鼠皮层Nrf2及HO-1表达变化的影响
Expression changes of Nrf2 and HO-1 in rat cortex following traumatic brain injury after hypox-ic preconditioning
摘要
Abstract
Objective To investigate the expression changes of nuclear factor erythroid 2-relat-ed factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in rat cortex following traumatic brain injury after hypoxic preconditioning. Methods Two hundred and sixteen adult male Sprague Dawley rats were randomly divided into control group (Con), hypoxic preconditioning group (HP), traumatic brain injury group (TBI) and hypoxic preconditioning + traumatic brain injury group (HP +TBI). Con and TBI groups had 12 rats respectively, TBI or HP + TBI group was subdivided into 8 groups according to sacrifice time points, namely group 1 h, 3 h, 6 h, 12 h, 24 h, 3 d, 7 d and 14d (n=12). TBI models were made by the Feeney’ s improved equipment and hypoxic preconditioning models were made by Hypobaric chamber for 3 days (-50kPa、3 h/d). The expression of Nrf2 and HO-1 in the brain cortex was detected by immunohistochemistry and western blotting. Results The expression of Nrf2 and HO-1 began to increase at 1 h, reached the peak at 24 h, and decreased slowly at 3 d after TBI. At the time points of 1 h to 7 d, the expression of Nrf2 and HO- 1 in the HP+TBI or TBI group was significantly higher than those in the Con or HP group ( p<0.05). After consecutive three days hypoxic preconditioning, the expression of Nrf2 and HO-1 had no significant difference between HP and Con groups, but the expression of Nrf2 and HO-1 in the HP+TBI group was higher than those in the TBI group from 1 h to 7 d (p<0.05). Conclusion Hypoxic preconditioning up-regulates the expression of Nrf2 and HO-1 in the brain cortex, which enhances the anti-oxidative ability of brain after TBI.关键词
颅脑损伤/核转录因子E2相关因子2/血红素加氧酶1/缺氧预处理Key words
Traumatic brain injury/Nuclear factor erythroid 2-related factor 2/Heme ox-ygenase-1/Hypoxic preconditioning引用本文复制引用
疏龙飞,刘家传,王金标,张永明,张星,杨艳艳,马涛,孙文江,卓健伟..缺氧预处理对颅脑损伤大鼠皮层Nrf2及HO-1表达变化的影响[J].中华神经创伤外科电子杂志,2015,(4):35-39,5.基金项目
全军医学科技“十二五”科研项目(面上)(CWS11J262) （面上）
2009年度南京军区医学科技创新重点课题(09Z009) （09Z009）
