中山大学学报（自然科学版）Issue(1)：1-11,11.DOI:10.13471/j.cnki.acta.snus.2016.01.001
多种细菌与凡纳滨对虾肝胰腺坏死症（HPNS）爆发有关
Multiple bacteria species were involved in hepatopancreas necrosis syndrome (HPNS)of Litopenaeus vannamei
摘要
Abstract
In recent years,a disease outbreak occurred in >50 percent of Litopenaeus vannamei farms at about 30 days after frys are released into the ponds in southern China.About 80% culture shrimps were dead in culture period.The clinical symptoms of the diseased shrimps include atrophy and necrosis of the hepatopancreas,weakened activity,and shrimp found dead in the bottom of the pond.The farmers in China usually called this symptom as “hidden death disease”.Since hepatopancreas atrophy and necrosis are the main symptoms of the disease,so we called this disease as hepatopancreas necrosis syndrome (HPNS).In March 2012 to October 2013,we have collected the diseased L.vannamei with the symp-toms of HPNS in 12 farms from Guangdong,Hainan and Guangxi provinces,China.Histopathology showed necrosis of hepatopancreas of the diseased shrimps,disappearance of some hepatopancreatic epi-dermal cells,and connective tissue.We used PCR to detect the presence of 11 viruses in 305 HPNS shrimps and performed artificial viral infection to healthy shrimps.The artificially infected shrimps did not show symptoms of HPNS.A total of 383 bacteria strains were identified from hepatopancreas,hemolymph and intestine of 63 diseased shrimps.These bacteria belong to 10 genera,49 species.We isolated Vibrio parahaemolyticus from 38 shrimps,Bacillus cereus from 34 shrimps,B.thuringiensis from 20 shrimps and V.cholera from 19 shrimps.Injection and immersion of V.parahaemolyticus and B.thuringiensis can cause symptoms of HPNS.The results suggest that multiple bacteria were involved in HPNS of L.van-namei.关键词
凡纳滨对虾/肝胰腺坏死症/细菌/病毒Key words
Litopenaeus vannamei/hepatopancreas necrosis syndrome (HPNS)/bacterium/virus分类
农业科技引用本文复制引用
黄志坚,陈勇贵,翁少萍,路晓锋,钟立洪,范文洲,陈旭凌,张慧文,何建国..多种细菌与凡纳滨对虾肝胰腺坏死症（HPNS）爆发有关[J].中山大学学报（自然科学版）,2016,(1):1-11,11.基金项目
国家基金联合基金资助项目（U1131002）；国家科技支撑资助项目（2012BAD17B03）；国家现代农业产业技术体系建设专项资助资金（CARS －47）；农业公益性行业科研专项经费资助项目（201103034）；广东省科技计划资助项目（2012A020602084）；广州市科技计划资助项目 （）
