滨州医学院学报2016，Vol.39Issue(1)：5-8,4.
川芎嗪联合阿糖胞苷对人白血病 U937细胞的增殖、凋亡影响及相关机制的研究
Inhibitory effect of combination treatment with tetramethylpyrazine and Cytarabine on proliferation and Apoplosis in U937 cell and its mechanism
摘要
Abstract
Objective To investigate the proliferation and cell apoptosis of combination treatment with tetramethylpyrazine (TMP)and Cytarabine (Ara-c)on human leukemia cell line U937 cell and provide experimental evidence and theoretical basis for finding effective chemotherapy sensitizer in clinical study.Methods The experiment was divided into TMP group,Ara-c group,TMP combined with Ara-c group,and the control group.The inhibitory rate of U937 cell proliferation was detected by CCK-8 assay.The apoptotic rate was examined by Flow cytometry (FCM).The expression of Bcl-2 and Bax of U937 cells were measured with immunohistochemical approach.Results TMP (0.5 mg·ml-1 )conbined with Cytarabine (0.02,0.04,0.08, 0.1 6 μg·ml-1 )did be able to inhibited the cell growth,compared with the Ara-c group in corresponding concentrations,the difference was significant(t=5.38,t=8.23,t=14.61,t =1 7.31,P <0.05).The inhibition of combination treatment with Tet-ramethylpyrazine (0.5 μg·ml-1 )and Cytarabine (0.08 μg·ml-1 )was significantly higher than Cytarabine (0.08 μg·ml-1 ) [(1 5.07±3.85)%],Tetramethylpyrazine (0.5 mg·ml-1 )[(3.1 7 ±2.20)%]and the control[(1.8 ±1.18)%],respectively (t=12.25,P <0.05;t=24.1 5,P <0.05;t=25.52,P <0.05).The result of Western blot show that the expression of Bcl-12 was up-regulated and the expression of Bax was down-regulated by Ara-c,to some extent.The expression levels of Bcl-2 and Bax were changed more significantly by combination treatment with TMP and Ara-c than the Ara-c alone (t=6.32,P <0.05,t=2.60,P <0.05;t=39.06,P <0.05,t=41.67,P <0.05).Conclusion Tetramethylpyrazine can apparently inhibit the growth of U937 cell and enhance apoptosis induced by cytarabine,promote the drug-sensitivity to cytarabine.And its molecular mecha-nisms might be related to down-regulation of the Bcl-2 expression and up-regulation of the Bax expression.关键词
U937 细胞株/川芎嗪/阿糖胞苷/细胞增殖/细胞凋亡/Bcl-2/BaxKey words
U937 cell line/tetramethylpyrazine/Cytarabine/cell proliferation/cell apoptosis/Bcl-2/Bax分类
医药卫生引用本文复制引用
唐慎华,贾秀红,李娜,王建勇..川芎嗪联合阿糖胞苷对人白血病 U937细胞的增殖、凋亡影响及相关机制的研究[J].滨州医学院学报,2016,39(1):5-8,4.基金项目
?基金项目:山东省自然科学基金(ZR2014HL032) （ZR2014HL032）
山东省医药卫生科技发展计划(2014WS0183) （2014WS0183）
滨州医学院校级课题(BY2011KJ016) （BY2011KJ016）
