中国妇幼健康研究2016，Vol.27Issue(1)：14-16,17,4.DOI:10.3969/j.issn.1673-5293.2016.01.005
高血糖通过HBP途径增加 SD大鼠子宫内膜细胞内β-catenin稳定性
Glucose enhances the stability ofβ-catenin via hexosamine biosynthetic pathway in endometrium of SD rats
周福兴 1陈颖 2刘高伟 1刘海霞 1李娜 1马芮 1陈必良1
作者信息
- 1. 第四军医大学西京医院妇产科，陕西西安710032
- 2. 第四军医大学药学院天然药物学教研室，陕西西安710032
- 折叠
摘要
Abstract
Objective To investigate the mechanism of hyperglycemia enhancing the stability of β-catenin in endometrial cells via hexosamin biosynthetic pathyway (HBP).Methods Female SD rats were randomly divided into 5 groups: normal control (NC) group, fasting ( FASTING) group, high sugar and high fat ( HSHF) group, glucosamine ( GLN) group and glucose ( GLU) group.Rats in the HSHF group were given high glucose and high fat diet , while the other groups were given normal diet .After 8 weeks’ feeding rats in HSHF group and NC group were executed and endometrium of them were collected .Rats in FASTING group were executed after 24h.GLN group and GLU group was lavaged with glucosamine (2.50g/kg) and glucose (5g/kg) after fasting for 24 hours.Three hours later the rats were executed.Then endometrium of rats in each gorup was collected to detect expressions of protein and genes .Results Compared with NC group, fasting blood glucose level increased remarkably in HSHF group (t value ranged 2.34 -2.78, both P<0.05).Compared with FASTING group, glucose level of GLU group enhanced significantly (t=3.46,P<0.05), but GLN group was not significantly different (t=0.84,P>0.05).Western blot detection results showed that the expressions of β-catenin and O-GlcNAc in GLN group and GLU group were remarkably higher than those in FASTING group (t value ranged 3.44-5.08, respectively, all P<0.05), but the differences in GLN group and GLU group were not significant (t value was 0.75 and 0.66, respectively, both P>0.05).RT-PCR detetion revealed that the difference in Ctnnb1 expression was not significant between HSHF group and NC group (t=0.43, P>0.05), but the expressions of CyclinD1 and Axin2 were remarkably higher in HSHF group than in NC group (t value was 4.48 and 5.26, respectively, both P<0.05). Conclusion Hyperglycemia can enhance O-GlcNAc level of β-catenin via HBP , and thus elevate its stability to help the accululation of β-catenin, which causes abnormal proliferation by consitent action on downstream target genes .关键词
高血糖/内膜癌/WNT/β-catenin/O-GlcNAcKey words
hyperglycemia/endometrial cancer/WNT/β-catenin/O-GlcNAc分类
医药卫生引用本文复制引用
周福兴,陈颖,刘高伟,刘海霞,李娜,马芮,陈必良..高血糖通过HBP途径增加 SD大鼠子宫内膜细胞内β-catenin稳定性[J].中国妇幼健康研究,2016,27(1):14-16,17,4.
