海南医学院学报2016，Vol.22Issue(9)：836-840,844,6.DOI:10.13210/j.cnki.jhmu.20160125.044
大肠癌组织中 KRAS 和 PIK3CA 基因突变关系的研究
Relationship of KRAS and PIK3CA gene mutation in colorectal cancer
摘要
Abstract
Objective:To analyze the relationship between KRAS/PIK3CA gene mutation and clinicopathologic charac-teristics such as gender,age,tumor location,pathological pattern,histological grade,TNM stage and lymph node metastasis, especially the relationship with distant metastasis of colorectal cancer.Methods:A total of 94 cases of colorectal cancer samples surgically resected in Gastrointestinal Surgery Department of our hospital from January 2012 to August 201 5 were collected, DNA was extracted and then KRAS and PIK3CA gene sequencing was carried out;their clinicopathologic characteristics (gen-der,age,tumor location,pathological pattern,histological grade,TNM stage,lymph node metastasis and distant metastasis) were analyzed,the relationship between KRAS/PIK3CA gene mutation and above factors,especially distant metastasis was an-alyzed,and statistical analysis processing was conducted;patients received 3-year follow-up,distant metastasis and recurrence were observed,and the number of their cases was counted,statistically analyzed and processed.Results:KRAS gene mutation was not associated with gender,age,tumor location,pathological pattern and histological grade (P >0.05),and significantly associated with distant metastasis,lymph node metastasis and TNM stage (P <0.05);PIK3CA was not associated with gen-der,age,tumor location,pathological pattern and histological grade (P >0.05),and associated with TNM stage,lymph node metastasis and distant metastasis (P <0.05);7 cases (7.4%)were with mutation of both KRAS and PIK3CA (double posi-tive),and 55 cases (57.4%)were with no mutation at all (double negative);in double positive cases,5 cases were with distant metastasis,metastasis rate was 71.4% and higher than that of double negative (1 6/55,29.1%),and there were statistical differences (P =0.039);it was found in follow-up that metastasis rate of KRAS mutant type was higher than that of wild type, and differences were statistically significant (P <0.05);recurrence rates of KRAS and PIK3CA mutant type were higher than those of wild type,and differences were statistically significant (P <0.05 ).Conclusions:KRAS and PIK3CA gene mutation rates in colorectal cancer tissue are associated with TNM stage,lymph node metastasis and distant metastasis,and mutant type is more susceptible to distant metastasis and recurrence than wild type;KRAS and PIK3CA gene mutation may have certain predictive value for distant metastasis and recurrence of colorectal cancer,and have certain guiding significance for judgment of patients’prognosis.关键词
大肠癌/KRAS 基因/PIK3CA 基因/基因突变/转移/复发Key words
KRAS gene/PIK3CA gene/Gene mutations/Colorectal cancer/Metastasis,Recurrence分类
医药卫生引用本文复制引用
姚凡保,邝倩仪,付汐,黄仕尧..大肠癌组织中 KRAS 和 PIK3CA 基因突变关系的研究[J].海南医学院学报,2016,22(9):836-840,844,6.基金项目
佛山市卫生和计生局医学科研课题(2015088)[Foundation Project]:It is supported by Health and Family Planning Bureau of Foshan (2015088)． （2015088）
