海南医学院学报2016，Vol.22Issue(10)：941-943,947,4.DOI:10.13210/j.cnki.jhmu.20160112.001
宫颈癌组织中 FoxM1及下游相关靶分子表达量的探究
Exploration of FoxM1 and downstream related target molecule expression in cervical cancer tissue
摘要
Abstract
Objective:To study the expression of FoxM1 and downstream related target molecules in cervical cancer tis-sue.Methods:Cervical cancer tissue and normal cervical tissue were collected to detect the expression of FoxM1,proliferation-related genes (CDK6 and CDK8)and angiogenesis-related genes (VEGFA,VEGFB and VEGFC);Hela cells were cultured and transfected with FoxM1 siRNA,and then expression of CDK6,CDK8,VEGFA,VEGFB and VEGFC were detected.Re-sults:mRNA contents of FoxM1,CDK6,CDK8,VEGFA,VEGFB and VEGFC in cervical cancer tissue were significantly higher than those in normal cervical tissue;mRNA content of FoxM1 was positively correlated with mRNA contents of CDK6, CDK8,VEGFA,VEGFB and VEGFC;mRNA contents of CDK6,CDK8,VEGFA,VEGFB and VEGFC of FoxM1-siRNA group were significantly lower than those of negative control-siRNA group.Conclusions:FoxM1 expression abnormally increa-ses in cervical cancer tissue,and its downstream target genes include CDK6,CDK8,VEGFA,VEGFB and VEGFC.关键词
宫颈癌/叉头框转录因子 M1(FoxM1)/增殖/细胞周期/血管新生Key words
Cervical cancer/Forkhead box M1/Proliferation/Cell cycle/Angiogenesis分类
医药卫生引用本文复制引用
袁毅翀,杨琼..宫颈癌组织中 FoxM1及下游相关靶分子表达量的探究[J].海南医学院学报,2016,22(10):941-943,947,4.基金项目
湖北自然科学基金(2012KFC143)[Foundation Project]:It is supported by Hubei Natural Science Foundation (No：2012KFC143)． （2012KFC143）
