吉林农业大学学报2016，Vol.38Issue(1)：38-44,7.DOI:10.13327/j.jjlau.2015.2754
桦褐孔菌内切葡聚糖酶基因的克隆与表达分析
Cloning and Expression Analysis of Endo-1,4-β-D-glucanase Gene of Inonotus obliquus
摘要
Abstract
Endoglucanase gene relative to fungal metamorphosis was first cloned from medicinal fun-gus Inonotus obliquus by rapid amplification of cDNA end PCR ( RACE) . The results showed that A 1 315 bp full-length cDNA of endoglucanase gene contained 1 233 ORF, which was predicted to en-code a 410-amino acid protein with 13 introns and 14 exons. Phylogenetic analysis showed that en-doglucanase gene of I.obliquus had the closest genetic relationship with EG of Fomitiporia Mediterra-nea. Quantitative real-time PCR analysis revealed that IO-EG expression was increasingly higher with the development of sclerotium of I.obliquus, suggesting that IO-EG was correlated with yield of scle-rotium of I.obliquus.关键词
桦褐孔菌/内切葡聚糖酶/菌核Key words
Inonotus obliquus/endoglucanase/sclerotium分类
农业科技引用本文复制引用
隋飞飞,李健,安丹丹,陈艳秋..桦褐孔菌内切葡聚糖酶基因的克隆与表达分析[J].吉林农业大学学报,2016,38(1):38-44,7.基金项目
国家自然科学基金项目(31160408) （31160408）
