吉林农业大学学报2016，Vol.38Issue(1)：87-91,5.DOI:10.13327/j.jjlau.2015.2715
神经生长因子mRNA在鹅等级前卵泡中的表达
Expression of Nerve Growth Factor mRNA in Pre-hierarchical Folli-cles of Geese
摘要
Abstract
In this study, pre-hierarchical follicles of Zi geese and Carlos geese were used as materi-als. By RT-PCR technique, expression of NGF in pre-hierarchical follicles of different periods was quantitatively studied, change of follicle stimulating hormone ( FSH) in two kinds of geese blood was tested, and relationship between content of FSH and expression of NGF was explored. The results were as follows:relative expression amount of NGF in primary follicles of Zi geese is very significant-ly higher than that of other models except that of the largest white follicles, which is in peek period ( P<0. 01 ) . Expression amount of NGF in pre-hierarchical follicles of Carlos geese is higher than that of middle white follicles ( P<0. 05) . For Zi geese, during the last stage of laying, expression a-mount of NGF in pre-hierarchical follicles is very significantly higher than that of middle white folli-cles ( P<0. 01) . For Carlos geese, expression amount of NGF in large white follicles is very signifi-cantly higher than that of other models ( P<0. 01) . Expression amount of NGF in primary follicles of Zi geese peek period, the last stage of laying and Carlos geese peek period is very significantly high-er than that of the last stage of Carlos geese ( P<0. 01) . Expression amount of NGF in small white follicles in Zi geese and Carlos geese show significantly positive correlation with expression amount of FSH in serum (P<0. 05). It shows a negative correlation with expression amount of FSH. And it shows a positive correlation with expression amount of FSH.关键词
神经生长因子/等级前卵泡/荧光定量PCRKey words
NGF/pre-hierarchical follicle/RT-PCR分类
农业科技引用本文复制引用
刘璐璐,隋玉健,朱海洋,武惠岩,杨辉,孙永峰..神经生长因子mRNA在鹅等级前卵泡中的表达[J].吉林农业大学学报,2016,38(1):87-91,5.基金项目
吉林省留学回国人员择优资助项目(2015),吉林省重点科技攻关项目(20150204025NY),科技部星火重点计划项目(2015GA660001),吉林省科技厅"三区"人才支持计划项目(2015),吉林省现代农业产业技术体系建设专项资金资助项目(201538) （2015）
