吉林农业大学学报2016，Vol.38Issue(1)：92-96,106,6.DOI:10.13327/j.jjlau.2015.2626
梅花鹿Thymosin beta 10基因RNAi慢病毒载体的构建及其对鹿茸干细胞增殖的影响
Construction of RNAi Lentiviral Vector for Thymosin beta 10 and Effects of Its Introduction on Proliferation of Antler Stem Cells in Si-ka Deer
摘要
Abstract
Thymosin beta 10 ( Tβ10) gene of antlerogenic periosteum cells of Chinese sika deer was interfered by using RNAi lentiviral vector system, and effects of the system introduction on prolifera-tion of antler stem cells were primarily studied. The results showed that: (1) Three sequences of small interfering RNAs, targeting Tβ10 gene of sika deer, were successfully ligated into the lentivi-ral plasmids (pLVTHM);(2) AP cells were successfully infected by recombinant lentivirus, which was obtained by each plasmid co-transfection into HEK 293t cells, together with the plasmids pS-PAX2 and pMD2. G;(3) The expression level of Tβ10 mRNA in the infected AP cells was signifi-cantly decreased through RT-PCR measurement, and the interference of Tβ10 played a role in the suppression of proliferation of AP cells through MTT assay. Therefore, in this study we successfully interfered Tβ10 gene in the AP cells with the efficiency of 35. 6% compared to the control, and ob-tained preliminary results for the role of Tβ10 in AP cells.关键词
鹿茸/Thymosinbeta10(Tβ10)/RNA干扰/增殖Key words
deer antler/Thymosin beta 10( Tβ10)/RNAi/proliferation分类
农业科技引用本文复制引用
张伟,褚文辉,路晓,董振,王权威,李春义..梅花鹿Thymosin beta 10基因RNAi慢病毒载体的构建及其对鹿茸干细胞增殖的影响[J].吉林农业大学学报,2016,38(1):92-96,106,6.基金项目
"十二五"国家高技术研究发展计划(863)项目(2011AA100603),国家自然科学基金项目(31170950),中国农业科学院特产研究所创新工程专项经费 （863）
