军事医学2016，Vol.40Issue(2)：113-116,4.DOI:10.7644/j.issn.1674-9960.2016.02.010
姜黄素对阿尔茨海默病模型大鼠学习记忆能力、海马结构及脑脊液中Aβ1-42和tau蛋白含量的影响研究
Effect of curcumin on abilities of learning and memory, morphological structure of hippocampus, cerebrospinal fluid concentration of Aβ1-42 and tau proteins in Alzhei-mer′s disease rat models
摘要
Abstract
Objective To investigate the effect of curcumin ( Cur ) on the ability of learning and memory , the morphological structure of hippocampus, cerebrospinal fluid (CSF) concentration of beta-amyloid (Aβ1-42) and tau proteins in Alzheimer′s disease ( AD) rat models .Methods Forty-five male Wistar rats with stable escape latency tested by Morris water maze were randomly divided into control group (Con), AD model group(AD) and Cur administration group(Cur) ( n=15 ) .D-galactose intraperitoneal injection was combined with Aβ25-35 hippocampus stereotactic injection to establish the AD rat models.The Cur group was administered with Cur ( Sigma, USA ) dissolved in DMSO and 0.9%NaCl at the concentration of 50 mg/ml, and the dose of Cur was determined according to their relative body mass (200 mg/kg).Rats in Con and AD groups were treated with the same volume of DMSO and 0.9%NaCl.Fourteen days after treatment , nine rats of each group were randomly selected to test learning and memory abilities by Morris water maze . Hippocampus morphological structure was observed under an optical microscope after hematoxylin and eosin dye (HE) staining.CSF was extracted from six rats in each group from the cistern through the foramen magnum using 1 ml syringe .Enzyme-linked immunosorbent assay (ELISA) was used to detect the CSF concentration of Aβ1-42 and tau proteins.Results Compared with the Con group, the average escape latency (AEL) of AD group was significantly longer (P<0.05 or P<0.01), the time spent in the quadrant was shorter (P<0.01), the number of crossings was smaller (P<0.01), more neurons were dark and pyknotic, CSF concentration of Aβ1-42was significantly lower (P<0.01), and tau protein was obviously higher (P<0.01).Compared with AD group, the AEL of Cur group was significantly shorter (P<0.05 or P<0.01), the time spent in the quadrant was longer (P<0.01), the number of crossings was smaller (P<0.05), fewer neurons were dark and pyknotic, CSF concentration of Aβ1-42 in the Cur group was notably higher (P<0.01) and tau protein was distinctly lower (P<0.01).Conclusion Cur can improve the learning and memory abilities of AD rats and attenuate the injury to hippocampus .The mechanism might be closely associated with the increase of the CSF concentration of Aβ1-42 and decrease of tau protein.关键词
姜黄素/阿尔茨海默病/学习和记忆/海马/脑脊髓液/Aβ1-42/tauKey words
curcumin/Alzheimer′s disease/learning and memory/hippocampus/cerebrospinal fluid/Aβ1-42/tau protein分类
医药卫生引用本文复制引用
徐志秀,喻超,陈俊俊,郝延辉,刘肖,高亚兵,赵黎,左红艳,彭瑞云..姜黄素对阿尔茨海默病模型大鼠学习记忆能力、海马结构及脑脊液中Aβ1-42和tau蛋白含量的影响研究[J].军事医学,2016,40(2):113-116,4.基金项目
国家科技重大专项资助项目(2014 ZX09J14101-04C) （2014 ZX09J14101-04C）
