军事医学2016，Vol.40Issue(2)：117-121,5.DOI:10.7644/j.issn.1674-9960.2016.02.011
模拟微重力对猕猴肺组织结构及HSP70表达的影响
Effect of simulated microgravity on tissue structure and HSP70 expression in lungs of rhesus macaques
许崇玉 1王萍 1陈杨 1朱敏立 1郑燕华 1蔡艺灵 1马华松 1陈志明1
作者信息
摘要
Abstract
Objective To investigate the effect of simulated microgravity on the tissue structure and the mRNA and pro-tein expression of HSP70 in lungs of rhesus macaques .Methods Fifteen healthy young male rhesus monkeys were randomly divided into 3 groups:control group (group A, n=5), 6 weeks′simulated microgravity group (group B, n=5) and recovery for 4 weeks after 6 weeks of simulated microgravity group (group C, n=5).-10 degree head-down-tilt was used to simulate microgravity .HE staining and transmission electron microscopy were used to observe the histopathological and ultrastructural changes in pulmonary tissues .The expression and location of HSP 70 in lung tissues were observed by immunohistochemistry (IHC) method.The mRNA and protein expression of HSP70 in lung tissues were detected by quan-titative real-time PCR ( Q-PCR) and Western blotting .Results Compared with group A , histopathological examination re-vealed alveolar septal thickening , partial fusion of pulmonary alveoli and alveolar and interstitial lymphocytic infiltration in groups B and C .Ultrastructure examination showed smaller and irregular nuclei of alveolar epithelial cells , an increased number and volume of the lamellar bodies , enhanced evacuation , part of vacuolar degeneration and mitochondrial swelling in groups B and C .IHC results showed that HSP 70 was expressed in bronchial epithelial cells , vascular endothelial cells and alveolar epithelial cells of pulmonary tissues in each group , either in cytoplasm or in nucleus .In addition , the alveolar interstitial and alveolar exudates in groups B and C also showed positive signals .Q-PCR and Western blotting results showed that HSP70 was expressed in all of the three groups , but no significant difference was found between any two groups (P>0.05).Conclusion Medium or long-term simulated microgravity can cause damage to the lung tissue of rhesus mon-keys, but it has no effect on the expression of HSP 70 in the lung tissue.关键词
失重/模拟微重力/猕猴/肺组织/损伤/HSP70Key words
weightlessness/simulated microgravity/rhesus macaque/lung/injury/HSP70分类
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许崇玉,王萍,陈杨,朱敏立,郑燕华,蔡艺灵,马华松,陈志明..模拟微重力对猕猴肺组织结构及HSP70表达的影响[J].军事医学,2016,40(2):117-121,5.
