检验医学与临床2016，Vol.13Issue(5)：586-587,2.DOI:10.3969/j.issn.1672-9455.2016.05.004
流式细胞术与 PCR-SSP 法检测 HLA-B27的比对及 HLA-B27亚型的研究
Comparison of HLA-B27 detection by flow cytometry and SSP-PCR and study on HLA-B27 subtypes
摘要
Abstract
Objective To investigate the application of flow cytometry (FCM ) and sequence‐specific primer polymerase chain reaction (PCR‐SSP) method in the detection of human leukocyte antigen‐B27 (HLA‐B27) and to observe the distribution of HLA‐B27 subtypes in the local region .Methods The HLA‐B27 level in 88 cases of sus‐pected ankylosing spondylitis (AS) was detected by FCM and PCR‐SSP .Results Among 88 cases of suspected AS , 82 cases were HLA‐B27 positive and 6 cases were HLA‐B27 negative by FCM ,the positive rate was 93 .2% ;whereas 83 cases were HLA‐B27 positive and 5 cases were HLA‐B27 negative by PCR‐SSP ,the positive rate was 94 .3% .In the subtyping results ,41 cases were B * 2705 genotype ,accounting for 49 .4% ,37 cases were B * 2704 genotype ,ac‐counting for 44 .5% ,1 case was B * 2702 genotype ,accounting for 1 .2% ,4 cases were positive without distinguished genotype .Conclusion The Kappa consistency coefficient is 0 .905 ,the detection results of HLA‐B27 by PCR‐SSP and FCM have higher consistency .The susceptible HLA‐B27 allele subtypes are mainly HLA‐B * 2704 and HLA‐B *2705 .关键词
强直性脊柱炎/HLA-B27/PCR-SSP/流式细胞术Key words
ankylosing spondylitis/HLA-B27/PCR-SSP/flow cytometry引用本文复制引用
白世杰,托娅,张保平,王丽娟,王雅菲..流式细胞术与 PCR-SSP 法检测 HLA-B27的比对及 HLA-B27亚型的研究[J].检验医学与临床,2016,13(5):586-587,2.基金项目
内蒙古自治区自然科学基金（2014MS0854）；内蒙古医科大学附属医院重大科研项目（NYFY-ZD2012015）。 （）
