检验医学与临床2016，Vol.13Issue(5)：600-601,603,3.DOI:10.3969/j.issn.1672-9455.2016.05.010
高危型人乳头瘤病毒 E2在宫颈癌中的表达及其生物学意义
Expression and biological significance of high risk human papillomavirus E 2 in cervical cancer
摘要
Abstract
Objective To study the expression and biological significance of high risk human papillomavirus (HPV) E2 in cervical carcinoma .Methods The gene microarray technology was adopted to detect HPV genotypes in the cervicitis tissues (56 cases) ,cervical intraepithelial neoplasia tissues (CIN ,55 cases) and cervical squamous carci‐noma tissues (58 cases) .The immunoblot assay was applied to detect the expression levels of high risk HPV E 2 pro‐tein in cervical tissues .The flow cytometry technology was used to detect the apoptosis of cervical cancer cells and the Transwell test was used to detect the cell invasion ability .Results The microarray analysis showed that the HPV16 and HPV18 infection positive rates in the cervical squamous carcinoma group were significantly increased compared with the cervicitis group ,while which in the CIN group had no significant change .The immunoblot results showed that the expression level of high risk HPV E2 protein in the cervical squamous carcinoma group was significantly de‐creased compared with the cervicitis group ,while which in the CIN group had no significant change .The apoptosis rate of cervical squamous cancer cell in the high risk HPV E 2 plasmid group was significantly increased compared with the empty carrier group ,while the invasion ability of cervical cancer cells was reduced significantly .Conclusion High risk HPV E2 protein owns the ability to induce apoptosis of cervical squamous cancer cells and is expected to be an effective target for the control and treatment of cervical cancer .关键词
高危型 HPV E2/宫颈癌/凋亡/侵袭Key words
high risk HPV E2/cervical cancer/apoptosis/invasion引用本文复制引用
罗晓辉,高玲娟,钟天鹰..高危型人乳头瘤病毒 E2在宫颈癌中的表达及其生物学意义[J].检验医学与临床,2016,13(5):600-601,603,3.基金项目
南京市科技发展计划项目（201308041）。 （）
