检验医学与临床2016，Vol.13Issue(5)：610-613,4.DOI:10.3969/j.issn.1672-9455.2016.05.014
17株耐亚胺培南肺炎克雷伯菌和大肠埃希菌相关耐药基因及分子流行病学分析
Analysis on molecular epidemiology and related drug-resistance genes of 17 strains of imipenem-resistant K .pneumoniae and E .coli
摘要
Abstract
Objective To investigate the status of molecular epidemiology and related drug‐resistance genes in 17 strains of imipenem‐resistant Klebsiella pneumoniae and Escherichia coli .Methods The carbapenemase pheno‐types were confirmed by the modified Hodge test ;PCR was adopted to detect the resistance genes of carbapenemase , AmpC and ESBLs ,then DNA sequencing was performed and the genotype was determined by internet comparison ;the homology and genetic correlation of isolated strians were detected and analyzed by the enterobacterial repetitive intergenic consensus PCR(ERIC‐PCR) and multiple locus sequence typing (MLST ) .Results KPC‐2 ,TEM‐1 ,SHV and CTX‐M genes were found in 15 strains of K .pneumoniae ,and SHV‐12 and CTX‐M‐24 were predominant geno‐types .KPC‐2 gene was detected in 2 strians of E .coli ,one of which also had TEM‐1 and CTX‐M‐24 gene and another had CMY‐42 and OXA‐1 gene .The carbapenemase genes of IMP ,VIM and NDM were not detected in 17 strains .The homology and genetic relationship analysis revealed that the 15 strains of K .pneumonias were divided into the three ERIC kinds of A ,B and C ,12 strains of class A were ST11 ,2 strains of class B were ST147 and ST290 ,and only 1 strain of C was ST967 ;2 strains of E .colis were the same ERIC kind .Conclusion The imipenem‐resistance of 17 strains is associated with KPC‐2 gene ,KPC‐2‐producing K .pneumoniae strains had clonal transmission and preva‐lence in the neurosurgery wards ,and ST11 type of K .pneumoniae was the predominant clone attributed to this out‐break ;17 strains were the strains of producing ESBLs or AmpC simultaneouly ;This is the first discovery of KPC‐producing K .pneumoniae of ST290 and ST967 in worldwide ;the control measures of nosocomial infection in clinical departments should be enhanced .关键词
肺炎克雷伯菌/大肠埃希菌/耐药基因/分子流行病学Key words
Klebsiella pneumoniae/Escherichia coli/drug-resistance gene/molecular epidemiology引用本文复制引用
洪宇,冯福英,郑宗富,曾锜,张薇,罗湘湘..17株耐亚胺培南肺炎克雷伯菌和大肠埃希菌相关耐药基因及分子流行病学分析[J].检验医学与临床,2016,13(5):610-613,4.基金项目
南京军区医药卫生科研项目（11MA118）；中国人民解放军第四七六医院院长基金项目（YZJJ201411）。 （）
