检验医学与临床2016，Vol.13Issue(5)：646-648,3.DOI:10.3969/j.issn.1672-9455.2016.05.027
耐万古霉素肠球菌的基因型和同源性分析
Study of genotypes and homology of vancomycin-resistant enterococcus
郭大敏 1黄汉 2廖康 2郭鹏豪 2陈怡丽2
作者信息
- 1. 广东省佛山市南海区第八人民医院检验科 528216
- 2. 中山大学附属第一医院检验科，广州 510080
- 折叠
摘要
Abstract
Objective To understand the genotypes and homology of vancomycin‐resistant enterococcus (VRE) isolated from the First Affiliated Hospital of Sun Yat‐sen University to provide the laboratory basis for clinical treat ‐ment ,prevention and control of VRE infection in this area .Methods The clinically isolated VRE strains were collect‐ed .Furthermore ,the minimal inhibitory concentrations (MIC) of vancomycin ,teicoplanin and linezolid were deter‐mined by the E‐test ;the vancomycin‐resistant genes were identified by the multiple‐PCR ,and the homology of VRE strains by the pulsed field gel electrophoresis (PFGE) .Results All 8 VRE strains ,identified as enterococcus faeci‐um ,were isolated from abdominal drainage fluid ,which were highly resistant to vancomycin (MIC ≥ 256 mg /L) ,me‐diate or resistant to teicoplanin ,and sensitive to linezolid ,tigecycline and tetracycline .The vancomycin‐resistant geno‐types were determined to be VanA‐type ;which were divided into the clone A and B by the PFGE classification ,in which 7 strains were the clone A and 1 strain was the clone B .Conclusion The 8 VRE strains carry the Van gene and are multidrug‐resistant ,has a small range prevalence in our hospital ,but is not the prevalence of single clone .关键词
万古霉素/耐药/肠球菌/基因型/同源性Key words
vancomycin/drug resistance/enterococcus/genotype/homology引用本文复制引用
郭大敏,黄汉,廖康,郭鹏豪,陈怡丽..耐万古霉素肠球菌的基因型和同源性分析[J].检验医学与临床,2016,13(5):646-648,3.
