山西中医学院学报2016，Vol.17Issue(1)：21-24,4.
两种肝郁脾虚型肠易激综合征动物模型制备方法比较
Comparison of two types animal IBS models with liver stagnation and spleen deficiency syndrome induced by different methods
摘要
Abstract
Objective:To compare and optimize the two types of animal IBS model producing processes with liver stagnation and spleen deficiency syndrome. One is to clip the tail irrigation plus gastric intake of rhubarb and the other one is restraint stress plus gastric intake of senna leaf. Methods:50 rats were randomly divided into blank group,large dosage modelⅠ,small dosage modelⅠ,large dosage and small dosage modelⅡ,groups with 5 females and 5 males in each group.Rats in modelⅠand modelⅡwere separately induced for liver stagnation. Rats in modelⅠwere irrigated by tail clipping plus gastric intake of rhubarb. Rats in modelⅡwere induced by restraintstress plus gastric intake of senna leaf. Compare rats behaviors,serum VIP and 5-HT,intestinalmocosa 5-HT and histopathological examination of distal colon in each group. Results:Compared with blank group,Serum VIP was increased in male rats in small and large dosage modelⅡgroups,decreased in female rats in large dosage of modelⅠgroup and female rats in small dosage of modelⅡgroup. Serum 5-HT:increase in male rats in small dosage of modelⅡ group.Area and IOD:in modelⅠandⅡgroups were significantly different (P<0.01). Histopathological examination of the colon showed that mild to medium swollen intestinal mocos,mild to medium infiltration of lymphocyteand eosinophile granulocyte in rats from controlled group. Those of rats in modelⅠandⅡgroups were not significantly different compared with controlled group. Conclusion:the optimized IBS animal model might be male rats of small dosage modelⅡ.关键词
肠易激综合征/肝郁脾虚/动物模型Key words
IBS/liver stagnation and spleen deficiency/animal model分类
医药卫生引用本文复制引用
陈富丽,窦志芳..两种肝郁脾虚型肠易激综合征动物模型制备方法比较[J].山西中医学院学报,2016,17(1):21-24,4.基金项目
国家自然科学基金资助项目 （）
