现代妇产科进展2016，Vol.25Issue(1)：6-9,4.DOI:10.13283/j.cnki.xdfckjz.2016.01.002
宫颈癌细胞株Hela中CSN6对E6-AP及p53的调节及其分子机制
The role of CSN6 in regulating E6AP and p53 in cervical cancer
张彭南 1郭奇桑 2宋昱 2刘艳梅 2高蜀君2
作者信息
- 1. 复旦大学附属妇产科医院妇科，上海 200011
- 2. 复旦大学附属妇产科医院宫颈疾病诊疗中心，上海 200011
- 折叠
摘要
Abstract
Objective:To explore the molecular mechanism of constitutive photomor-phogenesis 9 subunit 6 ( CSN6 ) on regulating E6 AP and p53 in cervical cancer cell line Hela cells. Methods:The expression of CSN6 was down-regulated by lentivirus-mediated in Hela cells. The expression of E6-AP was detected by Western blot. The expression of p53 downstream genes was detected by real-time PCR. The molecular mechanism of CSN6 regulated the protein expression of E6-AP was detected by transformations and ubiquitination experiments. Results:CSN6 decreased the turnover rate of E6 AP in the presence of the eukaryotic protein synthesis inhibitor cycloheximide(CHX). CSN6-mediated E6AP upregulation was enhanced by the pro-teasome inhibitor MG132 . Overexpression of CSN6 decreased the endogenous ubiquitination lev-el of E6AP in a dose-dependent manner. CSN6 knockdown caused an upregulation of p53 target gene expression,including GADD45,p21,Bax,etc,and reduced the growth of tumor in nude mice. Conclusion:CSN6 is a critical ubiquitination regulator of E6AP and can positively affect the steady-state expression of E6AP.关键词
宫颈癌/CSN6/E6AP/泛素化Key words
Cervical cancer/CSN6/E6 AP/Ubiquitination分类
医药卫生引用本文复制引用
张彭南,郭奇桑,宋昱,刘艳梅,高蜀君..宫颈癌细胞株Hela中CSN6对E6-AP及p53的调节及其分子机制[J].现代妇产科进展,2016,25(1):6-9,4.
