现代妇产科进展2016，Vol.25Issue(1)：10-13,18,5.DOI:10.13283/j.cnki.xdfckjz.2016.01.003
子痫前期血清通过Caveolin-1增加肾小球内皮细胞通透性
The high expression of caveolin-1 causes the hyperpermeability of human glomerular en-dothelial cells in preeclampsia patients
摘要
Abstract
Objective:To explore the serum impacts on the permeability of human renal glomerular endothelial cells ( HRGEC) in preeclampsia patients and the function of expressed caveolin-1 on the permeability of HRGEC. Method:To culture the primary HRGEC and observe the position of Caveolin-1 in HRGEC by immunofluorescence. Then culture HRGEC in a device called “transwell”. Three groups were divided:Group A was treated with the serum of normal pregnant when the cells overgrew the whole upper chamber. Group B was treated with the pre-eclampsia patients serum. Group C was treated with the same preeclampsia serum as the second group and also one caveolae inhibitor-Methyl-beta-cyclodextrinthe( MBCD) in a suitable con-centration . And then test the Evans-bule conjuncted BSA in the lower chamber to reflect the dy-namic permeability of HRGEC. Western blot was used to detect the quantity of caveolin-1 ex-pressed by HRGEC after the cells were stimulated by serums of each group for 24 hours. Re-sult:the permeability of HRGEC was significantly enhanced when they were dealt with pre-eclampsia serum and the inhibitor could reverse them partly(P<0. 05). The expression of cave-olin-1 was highly elevated ( P<0 . 05 ) , it was reduced when treated with MBCD ( P<0 . 05 ) . Conclusion:High expressed caveolin-1 in preeclampsia patients can lead to hyperpermeability of HRGEC,which may play an important role in proteinuria formation in preeclampsia patients.关键词
子痫前期/肾小球内皮细胞/Caveolin-1/屏障障碍/蛋白尿Key words
Preeclampsia/HRGEC/Caveolin-1/Barrier dysfunction/Proteinuria分类
医药卫生引用本文复制引用
徐钦洋,杜菲,蒋荣珍..子痫前期血清通过Caveolin-1增加肾小球内皮细胞通透性[J].现代妇产科进展,2016,25(1):10-13,18,5.基金项目
国家自然科学基金资助项目(No:81570444) （No:81570444）
国家科技部“十二五”支撑计划项目(No:2014BAI05B05) （No:2014BAI05B05）
上海交通大学医学院健康管理研究基金(LY201501) （LY201501）
