现代妇产科进展2016，Vol.25Issue(1)：42-46,5.DOI:10.13283/j.cnki.xdfckjz.2016.01.010
生物钟基因Period2对卵巢癌裸鼠移植瘤生长抑制作用的机制研究
Research on the mechanism of period2 on the growth inhibition of human ovarian cancer ;xenografts in nude mice
摘要
Abstract
Objective:To investigate the mechanism of biological clock gene Period2 on the growth inhibition of human ovarian cancer xenografts in nude mice. Methods:Construction of ovarian cancer xenograft in nude mice with ovarian cancer SKVO3 cell. By using gene trans-fection technique,the recombinant gene Period2 was introduced into the tumor tissue. The ex-pression of Period2 was detected by Real-time PCR and Western blot methods. The animals were sacrificed in two weeks after treatment and measured the weight of the tumor. The volume and weight of the tumor were measured during intervention. Real-time PCR and Western blot methods were used to detect the expressions changes of B cell lymphoma-2 gene ( Bcl-2 ) and Bcl-2 related X protein ( Bax ) gene in tumor tissues. Results:Period2 gene was successfully transfected and expressed in the tumor tissues of nude mice. Compared with the other two groups,the tumor growth rate of Period2 overexpression group was slowed, tumor volume and weight was reduced,tumor inhibition rate was increased,the expression of apoptosis gene Bax was increased,and the expression of apoptosis inhibition gene Bcl-2 was decreased. The differ-ence was statistically significant compared with the other two groups(P<0. 05). Conclusions:Period2 may promote tumor cell apoptosis by inhibiting the expression of apoptosis inhibition gene Bcl-2 and promoting the expression of apoptosis related gene Bax. So it can inhibit the growth and metastasis of ovarian cancer,and play a role in tumor suppression.关键词
卵巢癌/生物钟基因Period2/基因转染/B细胞淋巴瘤-2基因/Bcl-2相关X蛋白基因/细胞凋亡Key words
Ovarian cancer/Biological clock gene Period2/Gene transfection/B cell lymphoma -2(Bcl-2) gene/Bcl-2 related X protein(Bax) gene/Cell apoptosis分类
医药卫生引用本文复制引用
王朝霞,李莉,李风艳,张三元..生物钟基因Period2对卵巢癌裸鼠移植瘤生长抑制作用的机制研究[J].现代妇产科进展,2016,25(1):42-46,5.基金项目
山西省科技攻关项目(No:20120313019-1) （No:20120313019-1）
