中国动物传染病学报2016，Vol.24Issue(1)：1-6,6.
猫杯状病毒的分离鉴定及其全基因组的序列分析
SEQUENCE ANALYSIS OF THE COMPLETE GENOME OF FELINE CALICIVIRUS ISOLATE
摘要
Abstract
A feline calicivirus strain GX01-2013 was isolated by inoculating nasal swabs of diseased cats into MDCK cells. The complete sequence of the GX01-2013 strain was amplified in RT-PCR and analyzed. Its complete genome was 7704 bp in length. The nucleotide homology was 82.3%with HRB-SS strain. Phylogenetic analysis of capsid protein revealed that GX01-2013 was distant from the vaccine strains (F9, F4, 225 and 2024) as their overall similarity of amino acids ranged from 84.2% to 87.7%. In addition, no geographical difference was found among these strains. This study also revealed that four amino acid sites associated with virus neutralization altered in the E region (426-521aa) of the capsid gene.关键词
猫杯状病毒/全基因组/遗传进化分析Key words
Feline calicivirus/genome/phylogenetic analysis分类
农业科技引用本文复制引用
何平,祝健,韦祖樟,黄伟坚,陈樱,杨文娟,李法凯,赵卫锋,周华波,李瑞凯,梁明丽,韦红云,何剑桥..猫杯状病毒的分离鉴定及其全基因组的序列分析[J].中国动物传染病学报,2016,24(1):1-6,6.基金项目
广西教育厅高校科学技术研究项目（YB2014005）；广西大学科研基金项目（XJZ140236）；广西自然科学基金回国项目 （）
