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猫传染性鼻气管炎病毒的分离与鉴定

刘健 周锦萍 李鑫 徐锋 杨显超 邓波 杨德全 鞠厚斌 葛杰 龚国华

中国动物传染病学报2016,Vol.24Issue(1):22-26,5.
中国动物传染病学报2016,Vol.24Issue(1):22-26,5.

猫传染性鼻气管炎病毒的分离与鉴定

ISOLATION AND IDENTIFICATION OF FELINE INFECTIOUS RHINOTRACHEITIS VIRUSES

刘健 1周锦萍 1李鑫 1徐锋 1杨显超 1邓波 1杨德全 1鞠厚斌 1葛杰 1龚国华1

作者信息

  • 1. 上海市动物疫病预防控制中心,上海201103
  • 折叠

摘要

Abstract

Five isolates of feline infectious rhinotracheitis virus strains were identified in the present study using immunology, chemistry and biology methods. gD sequences had no mutations these isolates grew in F81 cells only and caused noticeable cell lesions. The viruses did not agglutinate red blood cells of cats, cocks, human o type, cattle, pigs, rabbits and sheep. The viral liability was sensitive to pH3.0, organic solvent and heating at 60℃. The plaque forming unit was determined to be 3×106PFU/mL, and TCID50 was 10-5.0TCID50/0.1mL. In conclusion, these five feline infectious rhinotracheitis viruses resembled with previously isolated strains.

关键词

猫疱疹病毒I型/分离/鉴定

Key words

Feline Herpesvirus type I/isolation/identification

分类

农业科技

引用本文复制引用

刘健,周锦萍,李鑫,徐锋,杨显超,邓波,杨德全,鞠厚斌,葛杰,龚国华..猫传染性鼻气管炎病毒的分离与鉴定[J].中国动物传染病学报,2016,24(1):22-26,5.

基金项目

上海市农业基础性项目(沪农科攻字(2014)第7-3-3号);上海市市级农口系统青年人才成长计划(沪农青字(2015)第2-8号) ()

中国动物传染病学报

OA北大核心CSTPCD

1674-6422

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