中国动物传染病学报2016，Vol.24Issue(1)：27-31,5.
H7N3亚型禽流感病毒反向遗传操作系统的建立
ESTABLISHMENT OF REVERSE GENETIC SYSTEM FOR H7N3 AVIAN INFLUENZA VIRUSES
摘要
Abstract
In the present study, the reverse genetics systems of A/duck/Zhejiang/690/2009(H7N3) (ZJ690) and A/duck/Zhejiang/766/2009/(H7N3) (ZJ766) were established. After 8 plasmids were co-transfected into 293T cells, two viruses were rescued and named as R-ZJ766 and R-ZJ690. Sequence analysis confirmed no unexpected mutations in the genome of two rescued viruses. To test the pathogenecity of wild-type and rescued viruses in mouse, each of 10 six-week-old BALB/c mice were inoculated intranasally with 106EID50 of viruses. Four viruses (ZJ690、ZJ766、R-ZJ690、R-ZJ766) did not kill any mice but all viruses caused weight loss. All viruses were recovered only from the nasal turbinates and lungs of the inoculated mice, suggesting the rescued viruses possessed the same biological characteristics as wild-type viruses. The establishment of the reverse genetics system of two H7 viruses would support the further study on the pathogenic and interspecies transmission of H7 Avian influenza virus.关键词
禽流感病毒/亚型H7N3/反向遗传操作系统Key words
Avian influenza virus/H7N3/reverse genetic system分类
农业科技引用本文复制引用
冯琳琳,滕巧泱,闫丽萍,李雪松,申之义,李泽君..H7N3亚型禽流感病毒反向遗传操作系统的建立[J].中国动物传染病学报,2016,24(1):27-31,5.基金项目
自然科学基金（面上）项目 （）
