中国动物传染病学报2016，Vol.24Issue(1)：38-43,6.
犬细小病毒SH14株VP2基因原核表达及免疫原性分析
PROKARYOTIC EXPRESSION AND CHARACTERIZATION OF VP2 OF CANINE PARVOVIRUS SH14 STRAIN
摘要
Abstract
According to the genome sequence of CPV SH14 strain, a pair of specific primers were designed for amplifying VP2 gene in PCR. The resulting PCR product was digested with BamHΙand XhoΙand cloned into pGEX-4T-1 vector to obtain the recombinant plasmid pGEX-4T-VP2. Then, pGEX-4T-VP2 was transformed into E.coli BL21 cells with IPTG induction. The expressed products were identified in SDS-PAGE. Polyclonal antibodies against CPV VP2 were prepared with the purified recombinant VP2 by vaccinating rabbits. The rabbit antibodies were used in Western blot and indirect immunofluorescence to detect their reactivity with the expressed VP2. The results showed that the recombinant VP2 expressed in E.coli BL21 cells reacted positively with rabbit polyclonal antibodies. The availability of the recombinant VP2 has laid the solid foundation for development of subunit vaccine, diagnostic methods and pathogenic mechanism research for CPV.关键词
犬细小病毒/VP2基因/原核表达/免疫原性Key words
Canine parvovirus/VP2 gene/prokaryotic expression/immunogenicity分类
农业科技引用本文复制引用
唐井玉,李传峰,宫晓华,李琦,丁明洋,陈宗艳,王桂军,刘光清..犬细小病毒SH14株VP2基因原核表达及免疫原性分析[J].中国动物传染病学报,2016,24(1):38-43,6.基金项目
国家自然科学基金（31270194、31300141）；农业公益性行业科研专项课题 （）
