中国动物传染病学报2016，Vol.24Issue(1)：52-56,5.
猪小肠抗菌肽Cecropin P1对猪呼吸道菌抑菌活性的研究
RESEARCH ON ANTIMICROBIAL ACTIVITY OF CECROPIN P1 TO PORCINE RESPIRATORY BACTERIA
摘要
Abstract
Antibacterial peptide cecropin P1 was originally isolated from the small intestines of the pigs. In the present study, the recombinant cecropin P1 produced in pichia pastoris was tested for its antibacterial activity against seven species of common swine respiratory bacteria including Streptococcussuis, Staphylococcusaureus, Pasteurella multocida, Actinobacilluspleuropneumoniae, swine Bordetella bronchiseptica, swine enteropathogenic E.coli and Streptococcus pyogenes. The recombinant cecropin P1 showed different degrees of bacteriostasis for these seven respiratory bacteria as measured by MIC (minimal inhibitory concentration). The most significant bacteristasis was observed for Streptococcus pyogenes, Pasteurella multocida and Staphylococcus aureus with MIC of 49.5 ug/mL, and moderate antibacterial activity for Sreptococcus suis and E.coli. In contrast, the recombinant cecropin P1 had the least bacteriostasis for swine Bordetella bronchiseptica as the MIC was 398 ug/mL. Currently, the vigorous use of antibiotics has caused the bacteria to have developed the high-level resistance. The availability of the recombinant cecropin P1 is of great significance as it possesses wide antimicrobial spectrum, toxin-free nature, high stability, less resistance and no residue in the body.关键词
猪小肠抗菌肽/抑菌活性/最小抑菌浓度Key words
Cecropin P1/antibacterial activity/minimum inhibitory concentration分类
农业科技引用本文复制引用
李伦锋,马志永,魏建超,谢春阳,彭帅,李蓓蓓,刘珂,邵东华,邱亚峰,史子学..猪小肠抗菌肽Cecropin P1对猪呼吸道菌抑菌活性的研究[J].中国动物传染病学报,2016,24(1):52-56,5.基金项目
国家重点基础研究发展计划(2014CB542703) （2014CB542703）
上海市科技兴农重点攻关项目（沪农科攻字（2013）第5-6号） （）
