中国动物传染病学报2016，Vol.24Issue(1)：57-61,5.
猪水肿病新型亚单位菌苗对小鼠的效力研究
PROTECTION OF A GENETIC SUBUNIT BACTERIN AGAINST PORCINE EDEMA DISEASE IN MOUSE MODEL
摘要
Abstract
The exotoxin SLT-IIeB of Escherichiacoli Ee strain was expressed in E.coli and purified. The recombinant protein was mixed with inactivated Ee strain and then emulsified with oil adjuvant to prepare a mixed subunit bacterin. Mice were immunized twice at week 0 and 2 and challenged intraperitoneally with virulent Ee strain at week 4. Antibodies were detected in ELISA and agglutination test. After the second immunization, antibody level increased obviously and the immune protection against virulent Ee strain was 70%and 91.7%in subunit bacterin group and whole bacterin group, respectively. In conclusion, the mixed subunit bacterin induced good protection against virulent Ee strain, which was promising for development of a highly efficacious vaccine against porcine edema disease.关键词
分泌毒素表达蛋白/亚单位菌苗/小鼠/保护力Key words
Porcine edema disease/exotoxin SLT-IIeB/subunit bacterin/protection分类
农业科技引用本文复制引用
胡利群,刘俞君,郭利伟,杨小林,刘国平..猪水肿病新型亚单位菌苗对小鼠的效力研究[J].中国动物传染病学报,2016,24(1):57-61,5.基金项目
国家863计划（2006AA10A206）；湖北省教育厅中青年重点项目 （）
