中国现代医学杂志2016，Vol.26Issue(4)：1-4,4.DOI:10.3969/j.issn.1005-8982.2016.04.001
右美托咪定预处理抑制脑缺血再灌注时谷氨酸的释放及其受体机制研究
Dexmedetomidine inhibit Glu release and NR1 expression in hippocampus on global cerebral ischemia-reperfusion rats
摘要
Abstract
Objective To investigate the effect of Dexmedetomidine on glutamate (Glu) release and NR1 expres-sion in hippocampus on global cerebral ischemia-reperfusion rats. Methods Ninety male Wistar rats were randomly divided into three groups; group S received sham operation; group I/R received ischemia-reperfusion; group D re-ceived Dexmedetomidine treatment before ischemia-reperfusion ( = 30). Global cerebral ischemia was induced ac-cording to Pulsinelli-Brierley method. In group D, Dexmedetomidine 3 μg/kg was injected through tail vein before ischemia, then 3 μg/(kg·h) with injection pump. Meanwhile equal volume saline administrated in group I/R animals. Combs was used to evaluate nerve function of rats; Glu levels were detected by HPLC in the rat hippocampus; NR1 expression in CA1 area was detected by immunohistochemistry method. Results Compared with group S, Balance beam and rope strength test scores in group I/R and D were significant decrease ( <0.05), and the level of Glu and NR1 were increase significantly ( < 0.05). Compared with group I/R, Balance beam and rope strength test scores in group D were significant decreased ( <0.05), and the level of Glu and NR1 were increased significantly ( <0.05). Conclusion Dexmedetomidine can reduce the rat global cerebral ischemia reperfusion injury and improve the nerve function defect, and the mechanism is related to the inhibition of Glu release and down regulate NR1 expression.关键词
右美托咪定/缺血/再灌注损伤/谷氨酸/N-甲基-D-天冬氨酸受体Key words
Dexmedetomidine/ischemia/reperfusion injury/glutamate/N-methyl-D-aspartate receptor分类
医药卫生引用本文复制引用
尚宇,关双成,李悦,高光洁..右美托咪定预处理抑制脑缺血再灌注时谷氨酸的释放及其受体机制研究[J].中国现代医学杂志,2016,26(4):1-4,4.基金项目
辽宁省自然科学基金资助项目 （）
