中国现代医学杂志2016，Vol.26Issue(4)：18-23,6.DOI:10.3969/j.issn.1005-8982.2016.04.004
不同剂量肝素对高糖诱导后的人腹膜间皮细胞水通道蛋白1表达的影响
Impact of different dosages' heparin on the expression of aquaporins 1 of human peritoneal mesothelial cells induced by high concentration glucose
李鸽 1赵久阳 2任泽银 1肖伟 1桂永丰1
作者信息
- 1. 湖南省常德市第一人民医院 肾病内科，湖南 常德，415000
- 2. 大连医科大学附属第二医院 肾内科，辽宁 大连 116000
- 折叠
摘要
Abstract
Objective To investigate the impact of different dosages' heparin on the expression of aquaporin-1 (AQP1) of human peritoneal mesothelial cells (HPMCs) induced by high concentration glucose (2.5 %W/W). Methods HPMCs were separated from patients and sub-cultured, and induced by high concentration glucose for 4, 8, 12, and 24 h respectively. This is to determinate the time when the mRNA of AQP1 reaches the maximum level. Heparin of different dosages and 2.5% glucose were added into the cell culture medium. The expression levels of AQP1 were detected by using RT-PCR and immunohistochemistry in both control and experimental groups. Results Compared with the control group, the mRNA expression level of AQP1 was up-regulated in an time-dependant manner, with the maximum expression at 24 h induced by high concentration glucose. The protein expression level of AQP1 was also up-regulated in all experimental groups, particularly by 2.5 % glucose and 1.5 í 10-2 mg/ml heparin. The dif-ference was of statistical significance ( <0.05). The result of RT-PCR is consistent with that of immunohistochem-istry. By using immunohistochemistry, vacuolation was observed in the nucleus and cytoplasm treated by 2.5 % glu-cose and 1.5 í 10-2 mg/ml heparin. Conclusion The mRNA and protein expression of AQP1 can be up-regulated by 2.5 % glucose and heparin. Vacuolation can be observed in the nucleus and cytoplasm treated by glucose and heparin of high concentration.关键词
肝素/人腹膜间皮细胞/水通道蛋白1Key words
heparin/human peritoneal mesothelial cells/aquaporin 1分类
医药卫生引用本文复制引用
李鸽,赵久阳,任泽银,肖伟,桂永丰..不同剂量肝素对高糖诱导后的人腹膜间皮细胞水通道蛋白1表达的影响[J].中国现代医学杂志,2016,26(4):18-23,6.
