中国现代医学杂志2016，Vol.26Issue(4)：58-63,6.DOI:10.3969/j.issn.1005-8982.2016.04.012
microRNA-221在结直肠癌中的表达及其对癌细胞迁移的影响
Expression of microRNA-221 in colorectal carcinoma and its impact on cancer cells' migration
王刚 1解寒冰2
作者信息
- 1. 河南省郑州人民医院急诊科，河南 郑州 450003
- 2. 河南省郑州人民医院普通外科，河南 郑州 450003
- 折叠
摘要
Abstract
Objective To study miRNA-221 expression in colorectal tissue and plasma of cancer patients, and to explore the mechanism about its migration of colorectal cancer. Methods 40 cases of colorectal cancer patients in our hospital as the study group,40 cases of healthy people as the control group, we used RT-PCR to detect the mR-NA levels in peripheral blood or tissue between of two groups; in addition, after overexpression or knockdown of mi-croRNA-221, we detected the migration of cancer cells. Results The expression of microRNA-221 in colorectal can-cer tissues was significantly higher than the adjacent tissues ( P< 0.05); in addition, the expression of microRNA-221 in plasma of colorectal cancer patients was significantly higher than the normal population ( P< 0.05). Knock-down of microRNA-221 in colorectal cancer cell line reduced cell invasion and E-cad expression significantly ( P<0.05);whereas overexpression of microRNA-221 in colorectal cancer cell line improved cell invasion and E-cad ex-pression significantly ( P<0.05). Conclusion High expression of microRNA-221 in colorectal cancer patients partic-ipated in the development of colorectal cancer by promoting the migration of colorectal cancer cells.关键词
microRNA-221/结直肠癌/上皮钙黏蛋白/迁移Key words
microRNA-221/colorectal cancer/E-cad/migration分类
医药卫生引用本文复制引用
王刚,解寒冰..microRNA-221在结直肠癌中的表达及其对癌细胞迁移的影响[J].中国现代医学杂志,2016,26(4):58-63,6.
