中国现代医学杂志2016，Vol.26Issue(5)：11-16,6.DOI:10.3969/j.issn.1005-8982.2016.05.003
划痕损伤对大鼠大脑皮层微血管内皮细胞NF-κB表达及继发性死亡的影响
Effects of scratching damage on NF-κB expression in brain microvascular endothelial cells and secondary death of rats
摘要
Abstract
Objective To culture brain microvascular endothelial cells (BMECs) of SD rats and construct a model of scratching injury, so as to observe the effect of scratching injury on NF-κB expression as well as auto-phagy, apoptosis and necrosis of of BMECs. Methods BMECs of SD rats were cultured and scratching injury was implemented, then the cells were treated with pyrrolidine dithiocarbamate (PDTC), an NF-κB inhibitor. The cells were randomly devided into control group, simple-injury group and injury-inhibition group. At 1, 6, 12, 24 and 48 hours after injury, NF-κB p65 expression was measured using immunofluorescent cytochemistry assay, apoptosis and necrosis of the cells were determined by Annexin V/PI staining, and expression of autophagy-related protein LC3 II was determined by Western blot. An uninjured group of BMECs was set as control. Results After scratching injury, NF-κB expression of the BMECs was enhanced from 1 h after injury, and peaked at 24 h, significantly higher than that of the control group ( <0.05). Indicators for apoptosis, necrosis and autophagy all increased to different extents as time went on, and they showed significant differences from those of the control group ( <0.05); while PDTC treatment following injury significantly inhibited these changes ( <0.05). Conclusions Scratching damage activates NF-κB expression of BMECs and causes autophagy, apoptosis and necrosis of BMECs.关键词
划痕损伤/脑微血管内皮细胞/NF- κB/吡咯醛二硫氨基甲酸Key words
scratching damage/BMEC/NF-κB/PDTC分类
医药卫生引用本文复制引用
黄梓雄,陈兵,聂丽容,林亨,莫伟,尹延庆,梁远生..划痕损伤对大鼠大脑皮层微血管内皮细胞NF-κB表达及继发性死亡的影响[J].中国现代医学杂志,2016,26(5):11-16,6.基金项目
2012年广东医学院附属医院青年科研基金（NoQk1308）；2013年广东医学院青年科研基金（NoXQ1315）；2014年度湛江市非资助科技攻关计划项目 （）
