中国现代医学杂志2016，Vol.26Issue(5)：22-25,4.DOI:10.3969/j.issn.1005-8982.2016.05.005
E3泛素连接酶Siah-1与糖尿病血管内皮损伤的关系研究
Relationship between E3 ubiquitin ligase SIAH1 and diabetic vascular endothelial injury
谷巍 1张雪坤 1赵正历 1孙殿静 1路玉李 1耿建林1
作者信息
- 1. 河北省衡水市哈励逊国际和平医院 内分泌科，河北 衡水 053000
- 折叠
摘要
Abstract
Objective To investigate the relationship between E3 ubiquitin ligase SIAH1 and diabetic vas-cular endothelial injury. Methods C57BL/6 rats and db/db rats were used in our study. Tension of isolated thoracic aorta was measured by organ bath and the vascular tension system. The levels of SIAH1 and β-catenin were detected by Western blot and ELISA. Results The vascular endothelial diastolic reaction induced by acetylcholine (Ach) in the db/db rats was significantly lower than that of the normal rats ( =24.270, =0.000). There was no significant difference in the vascular endothelial diastolic reaction induced by Ach be-tween the db/db rats using SIANH1 inhibitor and the normal rats ( =1.991, =0.327). There was no signifi-cant difference in the vascular endothelial diastolic reaction induced by sodium nitroprusside (SNP) between the db/db rats and the normal rats ( =0.145, =0.541). The level of SIAH1 decreased ( =5.483, =0.017) and that of β-catenin increased ( =6.670, =0.023) in the db/db rats using SIAH1 inhibitor. In the presence of β-catenin inhibitor, the vascular endothelial diastolic reaction of the diabetic rats using SIAH1 inhibitor had no significant difference from that of the normal rats ( =1.441, =0.378). Conclusions E3 ubiquitin ligase SIAH1 is involved in the process of vascular endothelial injury in diabetes, the mechanism may be related to its effect on β-catenin signal pathway.关键词
E3泛素连接酶Siah-1/糖尿病/血管内皮损伤Key words
E3 ubiquitin ligase SIAH1/diabetes/vascular endothelial injury分类
医药卫生引用本文复制引用
谷巍,张雪坤,赵正历,孙殿静,路玉李,耿建林..E3泛素连接酶Siah-1与糖尿病血管内皮损伤的关系研究[J].中国现代医学杂志,2016,26(5):22-25,4.
