中国药房2016，Vol.27Issue(7)：892-895,4.DOI:10.6039/j.issn.1001-0408.2016.07.09
鬼臼毒素衍生物QW-83对人宫颈癌HeLa细胞凋亡的影响及其机制研究
Study on Effects of Podophyllotoxin Derivative QW-83 on Human Cervical Cancer HeLa Cell Apoptosis and Its Mechanism
摘要
Abstract
OBJECTIVE:To study the effects of podophyllotoxin derivative QW-83 on human cervical cancer HeLa cell apopto-sis and its mechanism. METHODS:After treated with 0(negative control),0.01,0.1,1 and 10 μmol/L QW-83 and positive drug etoposide(VP-16)for 48 h,proliferation inhibition rate and IC50 of HeLa cell were determined by MTT assay. The morphological changes of HeLa cell were observed by Hochest 33342 staining after treated with QW-83 [0(negative control),2.5,5,10μmol/L] for 48 h;flow cytometry was used to detect apoptosis rate;semi quantitative RT-PCR was adopted to detect the expression of apop-tosis related gene P53,Bax,Casepase-3,Casepase-8,Casepase-9 and Bcl-2 mRNA. RESULTS:Compared with negative control, 1,10 μmol/L VP-16 and QW-83 had obvious proliferation inhibition effect on HeLa cells (P<0.05 or P<0.01),and IC50 were (5.11±0.43)μmol/L and(4.96±0.54)μmol/L. Hochest 33342 staining results showed QW-83 could obviously induce cells apopto-sis and nuclear pyknosis. Flow cytometry showed QW-83 could increase apoptosis rate in concentration-dependent manner,being 16.89%-62.56%. RT-PCR showed mRNA expression of P53,Bax,Caspase-3,Casepase-8 and Casepase-9,Bcl-2/Bax increased, while mRNA expression of Bcl-2 decreased after treated with QW-83(P<0.05). CONCLUSIONS:Podophyllotoxin derivative QW-83 can induce HeLa cell apoptosis,and its mechanism may be associated with regulate mRNA expression of apoptosis related gene.关键词
鬼臼毒素衍生物/QW-83/人宫颈癌HeLa细胞/抗肿瘤活性/凋亡相关基因/依托泊苷Key words
Podophyllotoxin derivative/QW-83/Human cervical cancer HeLa cell/Antitumor activity/Apoptosis-related gene/Etoposide分类
医药卫生引用本文复制引用
冷玲,高晨光,陈虹,牛聪,曹波..鬼臼毒素衍生物QW-83对人宫颈癌HeLa细胞凋亡的影响及其机制研究[J].中国药房,2016,27(7):892-895,4.基金项目
武警后勤学院中心实验室开放基金项目 （）
