中国药房2016，Vol.27Issue(7)：896-898,899,4.DOI:10.6039/j.issn.1001-0408.2016.07.10
坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响
Effects of Tamsulosin on Proliferation and Apoptosis in Prostatic Cancer PC-3 Cells
赵明 1金珊 1何锦华1
作者信息
- 1. 天津中医药大学第一附属医院泌尿外科，天津 300193
- 折叠
摘要
Abstract
OBJECTIVE:To explore the effects of tamsulosin on proliferation and apoptosis in prostatic cancer PC-3 cells. METHODS:After treated with 0 (blank control group),12.5,25 and 50 μmol/L tamsulosin (tamsulosin low,medium and high-concentration groups)for 48 h,the viability of PC-3 cells was detected by MTT method. Hoechst 33258 staining was used to detect cell apoptosis rate. Western blot was used to determine the expression level of Bax and Bcl-2 protein,and the phosphoryla-tion level of protein kinase B(Akt),mammalian target rapamycin(mTOR),ribosomal S6 protein kinase(p70S6K)and 4E bind-ing protein 1(4E-BP1). RESULTS:Compared with blank control group,PC-3 cells viability and the phosphorylation level of Akt, p70S6K and 4E-BP1 decreased in tamsulosin low,medium and high-concentration groups,while expression level of Bax protein in-creased (P<0.05 or P<0.01);the apoptosis rate of PC-3 cells was increased in tamsulosin medium and high-concentration groups,while the expression level of Bcl-2 and phosphorylation level of mTOR were decreased(P<0.01),in concentration-depen-dent manner. CONCLUSIONS:Tamsulosin can inhibit PC-3 cells proliferation and induce cell apoptosis via blocking Akt/mTOR signal pathway.关键词
坦索罗辛/前列腺癌PC-3细胞/增殖/凋亡/蛋白激酶B/哺乳动物雷帕霉素靶蛋白Key words
Tamsulosin/Prostatic cancer PC-3 cells/Proliferation/Apoptosis/Protein kinase B/Mammalian target rapamycin分类
医药卫生引用本文复制引用
赵明,金珊,何锦华..坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响[J].中国药房,2016,27(7):896-898,899,4.
