中国真菌学杂志2016，Vol.11Issue(1)：8-12,5.
黄芩苷联合氟康唑通过降低 cAMP 抑制白念珠菌酵母-菌丝形态转化
Effect of baicalin in combination with fluconazole against Candida albicans morphological transition from yeast to hyphae via inhibiting cAMP
摘要
Abstract
Objective This study aimed to investigate the antifungal activity of baicalin in combination with fluconazole a-gainst Candida albicans morphological transition from yeast to hyphae and to explore the mechanism.Methods The hypha-inducing liquid and solid media were uesd to observe the effect of baicalin alone or in combination with fluconazole on yeast-hapal transformation in C .albicans ;The intracellular cAMP level and cAMP rescue was measured by ELISA Kit;Quantitative real time PCR (qRT-PCR)was performed to measure the expression of cAMP-related genes.Results Baicalin in combination with fluconazole could inhibit hyphae formation and intracellular cAMP level obviously;exogenous cAMP could convert hy-pha-inhibiting effect;And expression of RAS1 and CDC35 were downregulated by 35% and 27% respectively,while the ex-pression of PDE2 was upregulated by 121%.Conclusion Mechanism of baicalin in combination with fluconazole inhibiting C . albicans morphological transition was probabaly via down-regulating the intracellular cAMP.关键词
白念珠菌/黄芩苷/氟康唑/形态转化/cAMPKey words
C .albicans/baicalin/fluconazole/morphological transition/cAMP分类
医药卫生引用本文复制引用
冯鑫,施高翔,云云,吴大强,邵菁,汪天明,汪长中..黄芩苷联合氟康唑通过降低 cAMP 抑制白念珠菌酵母-菌丝形态转化[J].中国真菌学杂志,2016,11(1):8-12,5.基金项目
安徽省自然科学基金项目(1408085MH165,1508085MH163) （1408085MH165,1508085MH163）
