广东海洋大学学报2016，Vol.36Issue(1)：13-18,6.DOI:10.3969/j.issn.1673-9159.2016.01.003
非诺贝特对卵形鲳鲹PPARα及CPTI基因表达的影响
Effects of Fenofibrate on Relatively Expression of Gene PPAR α and CPTI
摘要
Abstract
Thefenofibrate solution with concentration of 20, 50,100 mg/kg were injected into the abdomenofTrachinotus ovatus respectively, and the expression of genePPAR alpha andCPTIin livers and muscles were analyzed by RT-PCR, and the effects oftheagonistson relativelyexpression ofPPARαandCPTI gene was studied.Theresult showed that the expression ofPPAR α in group 50 mg/kg is significant higher than that in other groups(P<0.05), andCPTIhad higher expression in group 20mg/kg (P<0.05).The expression ofPPARαandCPTI gene had no significant difference in PBS group(P>0.05), but the expression ofPPAR α in group 50 mg/kgfirstly increased and decreased and thenreturned to the initial level trend finally as time changed.Meantime,the expression ofCPTIgenefell after rose and then rose at lastas the time gone by.The highest expression appeared at 30h afterinjection in livers and the highest expression appeared at 18h in muscles(P<0.05).Theexpression of genePPAR alphain livers and muscles withfenofibrate solution is higher than thatwith PBS.In conclusion,the fenofibrate can inducethe expression of PPAR α, but thecorrelation of the expression betweenPPAR α andCPTIisnot obviously.关键词
卵形鲳鲹/基因表达/PPARα/CPTI/非诺贝特/RT-PCR分析Key words
Trachinotus ovatus/gene expression/fenofibrate/PPAR α/CPTI/RT-PCR分类
生物科学引用本文复制引用
方玲玲,陈刚,王忠良,汤保贵,张健东,黄建盛,周晖..非诺贝特对卵形鲳鲹PPARα及CPTI基因表达的影响[J].广东海洋大学学报,2016,36(1):13-18,6.基金项目
国家海洋公益性行业科研专项（201205028）；广东省海洋经济创新发展区域示范专项（GD2012-A01-007，GD2012-A02-003）；广东省教育厅创新计划专项（2012KJCX0063）；广西科技厅科技计划 （）
