广东海洋大学学报2016，Vol.36Issue(1)：57-62,6.DOI:10.3969/j.issn.1673-9159.2016.01.010
溶藻弧菌肽聚糖和vp28-siRNA对凡纳滨对虾白斑综合征病毒的抑制作用
Effects ofVibrio alginolyticus Peptidoglycn andvp28-siRNA on Litopenaeus vannameiAgainstWhite Spot Syndrome Virus
摘要
Abstract
Vp28-siRNAat the concentration of 0.05and 0.10μg/μL was injected intoLitopenaeus vannameisufferedfrom White Spot Syndrome (WSS), respectively.The optimum concentration of vp28-siRNA was 0.10 μg/mL by interference effect experiment.L. vannamei waschallenged withWhite Spot Syndrome Virus (WSSV),andwas stimulated byvp28-siRNA at 24th day after infection.The effects ofvp28-siRNA interference at different times were detected. The results showed that the experimental group had statistical differences from the control group(P<0.05) at the6th hour,and WSSV copy numberhadarapiddecline at 48th hourand the good interference replication of WSSV lasted until the 120th hour. The protection rate was 40%.L. vannameiimmuned by peptidoglycan(0.1 μg/mL) was infected with WSSV. Thenvp28-siRNA was injected at the different times(6h,12h and 24h) into theshrimps and the protection rate was measured. The results revealed that the protection rateof group6h,12h and 24h was 80%,63.33%and 56.67%, respectively.It is more effective to interfere withWSSV so that it can reduce shrimp mortality, afterL. vannamei immuned with peptidoglycan andvp28-siRNA, and the earlierinterference, the higherrate of protection.关键词
凡纳滨对虾/免疫增强剂/RNA干扰/肽聚糖/vp28-siRNA/白斑综合征病毒Key words
Litopenaeus vannamei/immunopotentiating agent/RNA interference/peptidoglycan/vp28-siRNA/White Spot Syndrome Virus分类
农业科技引用本文复制引用
朱卫卫,邱德全,甘桢,鲁义善,简纪常..溶藻弧菌肽聚糖和vp28-siRNA对凡纳滨对虾白斑综合征病毒的抑制作用[J].广东海洋大学学报,2016,36(1):57-62,6.基金项目
广东省海洋渔业科技推广专项项目(A201100D03)，广东省海洋经济创新发展区域示范专项项目(GD2012-A03-012)，湛江市科技计划项目(2013A03021) （A201100D03）
