广东海洋大学学报2016，Vol.36Issue(1)：73-78,6.DOI:10.3969/j.issn.1673-9159.2016.01.013
高密度CO2对凡纳滨对虾肌球蛋白疏水性的影响
Effect of Dense Phase Carbon Dioxide on Hydrophobicity of Myosin Proteins fromLitopenaeus vannamei
摘要
Abstract
This study investigated the effect ofdense phase carbon dioxide (DPCD) on myosin gel formation induced by measuring the surface hydrophobicity and tyrosine intrinsic fluorescence spectrum of myosin in different conditions (pressure 5~30 MPa, temperature 50~70℃, time 10~50 min) with ANS probe and fluorescence scanning, respectively. The results indicated that DPCD treatment increased the surface hydrophobicity and the intrinsic fluorescence value of tyrosine significantly (P<0.05). Under the same temperature, the surface hydrophobicity and the intrinsic fluorescence value of tyrosine of myosin treated by DPCD were significantly higher than those of water bath heat-treatment (P<0.05). DPCD significantly enhanced the surface hydrophobicity and the intrinsic fluorescence value of myosin tyrosine with the increased pressure and temperature and the extension of time (P<0.05). These findings showed that DPCD could expose the hydrophobic group of myosin, which could loose the tight advanced structure.关键词
高密度CO2/肌球蛋白/表面疏水性/酪氨酸Key words
Dense phase carbon dioxide/myosin/surface hydrophobicity/tyrosine分类
轻工纺织引用本文复制引用
郭明慧,邓倩琳,刘书成,邓楚津,吉宏武,李承勇,高静..高密度CO2对凡纳滨对虾肌球蛋白疏水性的影响[J].广东海洋大学学报,2016,36(1):73-78,6.基金项目
国家自然科学基金资助项目(31371801)；国家虾产业产业技术体系专项基金(CARS-47) （31371801）
