广东海洋大学学报2016，Vol.36Issue(1)：93-97,5.DOI:10.3969/j.issn.1673-9159.2016.01.016
哈维氏弧菌qnr基因的克隆及原核表达条件优化
Cloning and Optimization of Prokaryotic Expression of Quinolone Resistance Gene in Vibrio harveyi
摘要
Abstract
The sequence of quinolone resistance gene ofVibrio harveyi was cloned based on theqnr of Vibrio harveyi published on GenBank and then inserted into the pET-32a vector to construct prokaryotic expression plasmid pET32-qnr.Then the expression conditions of temperature, induction time,and IPTG concentration of the pET32-qnr were optimized.The results showed that the completeopen reading frame (ORF) length ofqnrgene was 651bp,encoding a protein of 216 amino acids. The optimization conditions of inducible expression for Qnr protein wereinducingin 0.05 mmol/L IPTG for 6 hoursat 28℃.关键词
哈维氏弧菌/qnr基因/原核表达/优化Key words
Vibrio harveyi/quinolone resistance gene/prokaryotic expression/optimization分类
生物科学引用本文复制引用
周维,汤菊芬,高增鸿,甘桢,简纪常,吴灶和,丁燏..哈维氏弧菌qnr基因的克隆及原核表达条件优化[J].广东海洋大学学报,2016,36(1):93-97,5.基金项目
农业部行业专项，渔药使用风险评估及其控制技术研究与示范 （）
广东省教育厅高等学校高层次人才项目(谷胱甘肽及其合成酶系在哈氏弧菌耐药中的作用机制研究)。 （谷胱甘肽及其合成酶系在哈氏弧菌耐药中的作用机制研究）
